IMR Press / EJGO / Volume 22 / Issue 1 / pii/2001105

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Detection of numerical aberration in chromosome 17 and c-erbB2 gene amplification in epithelial ovarian cancer using recently established dual color FISH

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1 Department of Obstetrics and Gynecology, Hirosaki University School of Medicine, Hirosaki, Japan
Eur. J. Gynaecol. Oncol. 2001, 22(1), 23–25;
Published: 10 February 2001
Abstract

This paper presents a new dual color FISH technique that will allow the use of cell specimens isolated from formaldehyde-fixed and paraffin-embedded (F-P) tissues. The new FISH method was used to examine numerical aberration in chromosome 17 and c­erbB2 gene amplification in 26 F-P epithelial ovarian cancer tissues. Numerical aberration of chromosome 17 appears frequently in clear cell adenocarcinoma. The frequency of amplification of the c-erbB2 gene in stage III and IV cases was significantly higher than in stage I cases. The FISH technique as established here may serve as a molecular tool for examination of clinico-pathologi­cal significance in F-P tissues.

Keywords
Dual color FISH
Formaldehyde-fixed and paraffin-embedded tissue
Numerical aberration of chromosome 17
Ampli-fication of c-erbB2 gene
Epithelial ovarian cancer
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