IMR Press / EJGO / Volume 21 / Issue 5 / pii/2000220

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Clinical significance of numerical aberrations on chromosome 17 in uterine cervical and endometrial neoplasias

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1 Department of Obstetrics and Gynecology, Hirosaki University School of Medicine, Hirosaki, Japan
Eur. J. Gynaecol. Oncol. 2000, 21(5), 491–493;
Published: 10 October 2000
Abstract

In order to determine the clinical significance of numerical aberrations on chromosome 17 in uterine cervical and endometrial neoplasias, we investigated 140 cell specimens obtained from the uterine cervix and endometrium using the fluorescence in situ hybridization (FISH) method. These specimens consisted of ten normal cervical epithelium (NCE), 16 cervical intraepithelial neo­plasias (CIN 1), 15 CIN 2, 35 CIN 3, 11 early invasive squamous cell carcinoma of the uterine cervix (early invasive SCC), 11 invasive SCC, 13 normal endometrium (NE), 17 endometrial hyperplasias (EH), and 12 endometrial ademocarcinomas (EA). After Papanicolaou staining on these specimens was decolorized, FISH was performed using chromosome 17 specific repetitive DNA probes. Signals of centromere of chromosome 17 in marked atypical cells were counted using a fluorescence microscope. There was a signi­ficant difference in the rate of cells with one signal on chromosome 17 between CIN 1 (7.1 ± 0.7%) or 2 (7.0 ± 0.5%) and CIN 3 (12.6 ± 0.9%) (p < 0.01), and also between CIN 3 and early invasive SCC (19.6 ± 1.0%) (p < 0.01). The rate of cells with three signals was significantly increased when the uterine cervical lesions were progressive to CIN 3 (p < 0.01). Cells with five or more signals occurred only in early invasive SCC and invasive SCC. There was a significant difference in the rate of cells with three signals between EH (4.8 ± 0.6%) and EA (11.4 ± 2.1 %) (p < 0.05). Cells with five or more signals occurred only in EA. Examination of the numerical aberrations on chromosome 17 in uterine cervical and endometrial neoplasias has been suggested to be useful as an additional method for the differential diagnosis of these lesions.

Keywords
Chromosome 17
Numerical aberration
Fluorescence in situ hybridization
Uterine cervical neoplasia
Endometrial neo-plasia
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