IMR Press / EJGO / Volume 20 / Issue 1 / pii/1999113

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Establishment and growth regulation of a novel ovarian cancer cell line from a poorly-differentiated adenocarcinoma: proposal for a new treatment

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1 Experimental Oncology Department B. National Tumour Institute, "Fondazione G. Pascale", Naples, Italy
2 Hematology and Genetics Lah. “Casa Sollievo della Sofferenza”, S. G. Rotondo, Italy
3 Institute of Pathology and Animal Health, Veterinary Medicine, University of Naples “Federico II”, Naples, Italy
4 Hematology “ Laboratory,Cardarelli” Hospital, Naples, Italy
5 Gynaecology Institute, University of Padua, Italy
Eur. J. Gynaecol. Oncol. 1999, 20(1), 45–52;
Published: 10 February 1999

A continuously growing cultured cell line has been obtained in vitro, starting from a specimen of ascites fluid obtained from a patient with ovarian cancer, in whom a poorly-differentiated adenocarcinoma was diagnosed. This cell line, named OC-A I, is rou­tinely grown in standard, serum-supplemented culture medium and has been fully stabilized to long-term growth and characterized for both cultural and genetic parameters. OC-A I cells express a set of characteristics, as determined in vitro which, when compa­red with the in vivo primary tumor, confirm the high malignity of this cancer. In addition, karyotype analysis showed a translocation of chromosome 8 which is correlated with the amplification of c-myc oncogene. However, the expression of this oncogene was found to be significantly inhibited by a new regulatory activity, recently found to be present in a liposarcoma cell line. Conditioned medium from these cells was indeed able to inhibit the growth of OC-A I cells, arresting their cell cycle in the G1 phase and inducing them to apoptosis. Finally, the cell programmed death appeared to be related to the expression of antioncogene p53.

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