IMR Press / EJGO / Volume 20 / Issue 1 / pii/1999113

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Establishment and growth regulation of a novel ovarian cancer cell line from a poorly-differentiated adenocarcinoma: proposal for a new treatment

Show Less
1 Experimental Oncology Department B. National Tumour Institute, "Fondazione G. Pascale", Naples, Italy
2 Hematology and Genetics Lah. “Casa Sollievo della Sofferenza”, S. G. Rotondo, Italy
3 Institute of Pathology and Animal Health, Veterinary Medicine, University of Naples “Federico II”, Naples, Italy
4 Hematology “ Laboratory,Cardarelli” Hospital, Naples, Italy
5 Gynaecology Institute, University of Padua, Italy
Eur. J. Gynaecol. Oncol. 1999, 20(1), 45–52;
Published: 10 February 1999
Abstract

A continuously growing cultured cell line has been obtained in vitro, starting from a specimen of ascites fluid obtained from a patient with ovarian cancer, in whom a poorly-differentiated adenocarcinoma was diagnosed. This cell line, named OC-A I, is rou­tinely grown in standard, serum-supplemented culture medium and has been fully stabilized to long-term growth and characterized for both cultural and genetic parameters. OC-A I cells express a set of characteristics, as determined in vitro which, when compa­red with the in vivo primary tumor, confirm the high malignity of this cancer. In addition, karyotype analysis showed a translocation of chromosome 8 which is correlated with the amplification of c-myc oncogene. However, the expression of this oncogene was found to be significantly inhibited by a new regulatory activity, recently found to be present in a liposarcoma cell line. Conditioned medium from these cells was indeed able to inhibit the growth of OC-A I cells, arresting their cell cycle in the G1 phase and inducing them to apoptosis. Finally, the cell programmed death appeared to be related to the expression of antioncogene p53.

Share
Back to top