IMR Press / EJGO / Volume 19 / Issue 3 / pii/1998152

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Concurrent cytogenetic and molecular investigations in uterine and ovarian neoplasms

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1 General Biology and Medical Genetics, University of Pavia, Italy
2 General Biology and Medical Genetics, University of Pavia, Italy
3 Pathology Institute, IRCCS S. Matteo Polyclinic, Pavia, Italy
Eur. J. Gynaecol. Oncol. 1998, 19(3), 234–238;
Published: 10 June 1998
Abstract

We studied a group of 24 uterine and ovarian neoplasms with the purpose to verify if any correlation could be established between chromosomal abnormalities, loss of heterozigosity (LOH) and microsatellite instability (MIN). Tumor specimens obtained from 24 women (12 affected by ovarian and 12 by uterine neoplasms) were split in two parts, one was used for short term cultures for cytogenetic investigation while from the second DNA was extracted for molecular studies. We studied 22 polymorphic loci from 19 chromosomes and compared the alleles observed in the tumor with those observed in the DNA obtained from peripheral blood. Extensive loss of heterozigosity was observed when total or partial chromosomal loss was obser­ved in at least 50% of the examined cells; MIN did not correlate with any particular cytogenetic abnormality nor with LOH.

Keywords
Uterine and ovarian cancers
Chromosome abnormalities
Microsatellite instability (Ml)
Loss of heterozygosity (LOH)
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