IMR Press / EJGO / Volume 18 / Issue 5 / pii/1997159

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

The presence of HPV in cervical biopsies, determined by histology and PCR with 2 different pairs of consensus primers

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1 Department of Surgery A, Fars? Sygehus; Royal Dental College, Aarhus, Denmark
2 Institute of Pathology, Aarhus University Hospital, Royal Dental College, Aarhus, Denmark
3 Department of Maxillo-Facial Surgery and Oral Pathology, Royal Dental College, Aarhus, Denmark
Eur. J. Gynaecol. Oncol. 1997, 18(5), 365–367;
Published: 10 October 1997
Abstract

The link between human Papillomavirus (HPV) and cervical carcinomas has become increasingly convincing recently, and the detection of infection with certain HPV types may become an important element in screening for cervical pre-cancer. We applied the polymerase chain reaction (PCR) technique to detect and type HPV-DNA in biopsies from the uterine cervix with a histological dia­gnosis of CIN and condylomatosis. Forty-eight consecutive cervical biopsies were analysed for HPV-DNA by PCR, using two diffe­rent sets of consensus-primers. Typing was performed with HPV type-specific primers. Histological reevaluation revealed that 42 biopsies (87%) had condylomatous features. HPV was demonstrated in 46/48 (95.8%). HPV type 6/ 11 was found in seven biopsies, while HPV 16 was present in 23 samples, including two cases of double-infection. HPV type 18 was not found at all, and in 19 cases the HPV type present could not be determined. Apparently, HPV-DNA was absent in two cases. The results indicate that HPV-DNA screening is a valuable supplement to histopathological screening for cervical neoplasia. The value of different primer pairs is discussed.

Keywords
HPV
Cervical intraepithelial neoplasia (CIN)
Polymerase chain reaction (PCR)
Koilocytosis
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