IMR Press / EJGO / Volume 18 / Issue 1 / pii/1997121

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Detection of K-ras mutations in cancerous lesions of human endometrium

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1 Department of Gynaecological Surgery, University School of Medicine, Lublin, Poland
2 Department of Physiological Chemistry, University School of Medicine, Lublin, Poland
3 Department of Human Genetics, University School of Medicine, Lublin, Poland
Eur. J. Gynaecol. Oncol. 1997, 18(1), 80–83;
Published: 10 February 1997
Abstract

The many genetic changes associated with human carcinogenesis include the activation and/or inactivation of various genes. Poly­merase-chain reaction and single-strand conformation polymorphism analysis [PCR-SSCP] was used to detect alterations at exon 1 of the K-ras gene in 20 lesions of human endometrium. Six cases of endometrial hyperplasia, 13 of endometrial carcinoma and one of endometrial metastasis of ovarian cancer were analyzed. Mutations at exon 1 of the K-ras gene were detected in two of 13 human endometrial carcinomas [15%]; both were histologically defined adenocarcinomas, stage lb and stage Ila according to the FIGO. Alterations were also observed in the single case of endometrial metastasis of ovarian cancer. It is worthy of note that among the six women with hyperplasic endometrial lesions K-ras gene mutation were not reported. These data suggest that K-ras activation is rare in Polish women and when it does occur it is in cancerous, but not in precancerous, lesions of human endometrium.

Keywords
K-ras
Endometrial hyperplasia
Endometrial cancer
PCP-SSCP
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