IMR Press / CEOG / Volume 48 / Issue 4 / DOI: 10.31083/j.ceog4804138
Open Access Original Research
Ex vivo myolysis with dual wavelengths diode laser system: macroscopic and histopathological examination
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1 Department of Surgical Sciences, Division of Obstetrics and Gynecology, University of Cagliari, 09124 Cagliari, Italy
2 Department of Medical Sciences and Public Health, Unit of Anatomic Pathology, University of Cagliari, 09124 Cagliari, Italy
3 Department of Obstetrics and Gynecology, “Filippo del Ponte” Hospital, University of Insubria, 21100 Varese, Italy
4 Department of Women and Children’s Health, Clinic of Gynecology and Obstetrics, University of Padua, 35121 Padua, Italy
5 Department of Medical and Surgical Sciences, Institute of Obstetrics and Gynaecology, University of Foggia, 71121 Foggia, Italy
*Correspondence: (Maurizio Nicola D'Alterio)
Clin. Exp. Obstet. Gynecol. 2021, 48(4), 875–882;
Submitted: 7 March 2021 | Revised: 28 March 2021 | Accepted: 9 April 2021 | Published: 15 August 2021
Copyright: © 2021 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license (

Background: Uterine fibroids are the most common benign gynaecological tumours in women. When symptomatic, heavy menstrual bleeding is the principal manifestation of uterine fibroids. Nowadays, several conservative procedures have been utilized to discourage hysterectomy or myomectomy and great interest has been focused on laser energy technology associated with mini-invasive approaches. Diode lasers demonstrated many advantages in laparoscopic and hysteroscopic surgery. Methods: To evaluate the Dual Wavelength Diode Laser System (DWLS) ablation techniques for treating fibroids, we devised an experimental system for characterizing the myolysis fibre ablation performance with a particular fibre designed to create a thermal ablation in the centre of the myoma. All the myomas were used for the experiment after a total hysterectomy and their excision from the fresh uteri. The experiment setup was composed of two beaker containers filled with isotonic saline water, temperature monitoring myoma surface and water bath, a diode laser fibre and a diode laser energy deposition system. Results: Good macroscopic results have been found for fibroids of about 60 cm3 ablated with a setting of 10 W of 980 nm + 10 W of 1470 nm and for samples of about 15 cm3 ablated with a setting of 5 W of 980 nm + 5 W of 1470 nm, where the volume of the ablated fibroids were 46.6% and 41.6%, respectively. Conclusion: This is the first ex vivo study with a new DWLS, which made it possible to understand which one of its settings could be recommended for the safest and best macroscopic results in myolysis.

Uterine fibroids
Diode laser
Ex vivo
Fig. 1.
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