IMR Press / JOMH / Volume 17 / Issue 4 / DOI: 10.31083/jomh.2021.072
Open Access Original Research
ATF3 inhibits the growth and stem cells-like features of SW620 colorectal cancer cells in vitro
Show Less
1 Department of Medical Oncology and Radiotherapy, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China
2 Department of Gastroenterology, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China
3 Department of Hematology and Rheumatism, Ningde Municipal Hospital Affiliated to Ningde Normal University, 352000 Ningde, China
J. Mens. Health 2021, 17(4), 287–294; https://doi.org/10.31083/jomh.2021.072
Submitted: 6 April 2021 | Accepted: 8 June 2021 | Published: 30 September 2021
Abstract

Background and objective: Activating transcription factor 3 (ATF3) plays a crucial role in regulating tumor development depending on the cellular context or cancer cell type. However, the effect of ATF3 on stem cells-like features in colorectal cancer (CRC) has yet to be elaborated.

Methods: In this study, we overexpressed ATF3 in SW620 CRC cells to investigate its effects on stem cells-like features.

Results: Our results indicated that overexpressing ATF3 inhibited the proliferation, invasion, migration, and sphere formation capacity of SW620 CRC cells. ATF3 overexpression also decreased the size of tumorspheres and reduced expression of the cancer stem cell markers CD44 and CD133 in SW620 cells.

Conclusion: In summary, our study revealed that ATF3 suppresses CRC growth and stem cells-like features. ATF3 is considered a potential target in CRC therapy.

Keywords
ATF3
Migration
Invasion
Cancer stem cells
Figures
Fig. 1.
Share
Back to top