IMR Press / JOMH / Volume 15 / Issue 2 / DOI: 10.22374/jomh.v15i2.144

Journal of Men’s Health (JOMH) is published by IMR Press from Volume 17 Issue 1 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Dougmar Publishing Group.

Open Access Original Research


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1 Department of Surgery, College of Medicine, Prince Sattam bin Abdulaziz University, Al-Kharj, Saudi Arabia
2 Discipline of Pathology, School of Medical Sciences, Sydney Medical School, University of Sydney, Sydney, Australia
3 Lahore Institute of Fertility and Endocrinology (LIFE), Lahore, Pakistan
4 Department of Environmental Health Sciences, University of Massachusetts, Amherst, Massachusetts, USA
5 Faculty of Medicine, Department of Anatomy, Al-Azhar University, Cairo, Egypt

*Author to whom correspondence should be addressed.

J. Mens. Health 2019, 15(2), 58–65;
Submitted: 9 April 2019 | Accepted: 27 May 2019 | Published: 17 June 2019

Background and objective

The aim of the present study was to identify the impact of defective standard sperm parameters indi-vidually and in combination on DNA damage in a large cohort of infertile men.

Material and methods

Retrospective analysis of semen characteristics was conducted on 436 patients. DNA fragmentation analysis was performed by using the terminal deoxynucleotidyl transferase (TdT)-mediated fluoresce-in-dUTP nick end labeling (TUNEL) assay. Sperm parameters were arranged into different categories such as normospermia asthenospermic, teratospermic, asthenoteratospermic, and oligoasthenoter-atospermic. GraphPad Prism version 7 software was used for data analysis.


Our results suggest that the mean percentage of DNA damage was proportionally higher than the semen abnormalities. Sperm with 3 abnormal parameters showed significantly higher DNA damage, suggesting that sperm having more than 2 abnormalities are more likely to have higher DNA damage.


Sperm motility had significant correlation and is supposed to be a predictor for these tests, while mor-phology was the second standard sperm parameter inversely correlated with sperm DNA damage. Patients demonstrating low levels of leukocytospermia should be advised sperm DNA testing before assisted reproductive technology treatment. However, there is a clear need for more research studies to further address these issues.

semen analysis
DNA fragmentation
male infertility
sperm abnormalities
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