Imaging of Evoked Cortical Depolarizations Using Either ASAP2s, or chi-VSFP, or Di-4-Anepps, or Autofluorescence Optical Signals

Background : Population voltage imaging is used for studying brain physiology and brain circuits. Using a genetically encoded voltage indicator (GEVI), “VSFP” or “ASAP2s”, or a voltage-sensitive dye, Di-4-Anepps, we conducted population voltage imaging in brain slices. The resulting optical signals, optical local field potentials (LFPs), were used to evaluate the performances of the 3 voltage indicators. Methods : In brain slices prepared from VSFP-transgenic or ASAP2s-transgenic mice, we performed multi-site optical imaging of evoked cortical depolarizations - compound excitatory postsynaptic potentials (cEPSPs). Optical signal amplitudes ( ∆ F/F) and cEPSP decay rates (OFF rates) were compared using analysis of variance (ANOVA) followed by unpaired Student’s t test (31–104 data points per voltage indicator). Results : The ASAP2s signal amplitude ( ∆ F/F) was on average 3 times greater than Di-4-Anepps, and 7 times greater than VSFP. The optical cEPSP decay (OFF rate) was the slowest in Di-4-Anepps and fastest in ASAP2s. When ASAP2s expression was weak, we observed slow, label-free (autofluorescence, metabolic) optical signals mixed into the ASAP2s traces. Fast hyperpolarizations, that typically follow depolarizing cortical transients (afterhyperpolarizations), were prominent in ASAP2s but not present in the VSFP and Di-4-Anepps experiments. Conclusions : Experimental applications for ASAP2s may potentially include systems neuroscience studies that require voltage indicators with large signal amplitude ( ∆ F/F), fast decay times (fast response time is needed for monitoring high frequency brain oscillations), and/or detection of brain patches in transiently hyperpolarized states (afterhyperpolarization).


Introduction
Functioning neurons generate electrical fields, which are best studied by electrodes [1].However, voltage imaging can also be used to monitor neuronal electrical fields [2,3], especially when large areas are under investigation [4][5][6][7][8], or when the voltage imaging is done through an intact animal skull [9].
Genetically encoded voltage indicators (GEVI) have emerged as a valuable tool in experimental neuroscience [10][11][12][13].The performance of newly developed GEVIs vary between laboratories, preparations, and applications [14,15].Previously, we developed the GEVI, ASAP1 with a four-helix voltage-sensing domain.We mutated the positively charged residues in the fourth helix (S4) responsible for sensing the transmembrane electrical field.The resulting GEVI variant, ASAP2s, contained the R415Q mutation, which neutralizes one of the sensing charges in S4.ASAP2s showed an improved voltage responsiveness, slower offrate than ASAP1, and compatibility with two-photon imaging applications [16].In the current study, we explore the properties of ASAP2s for population voltage imaging.
Wide-field population voltage imaging methods lack cellular resolution.Mixed synaptic and action potentials arising from hundreds of dendrites and axons arrive onto the same optical detector.Such optical signal represents the "mean" response of many neurons.In population imaging experiments, the activity of many elements, such as desynchronized activations of hundreds of neurons, is mixed into one representative signal, a signal that represents a given population of cells projected onto the optical detector [17][18][19][20][21][22].
Using side-by-side measurements, including the same light source, optical path, detector, and stimulation paradigm, we compared three voltage indicators: two GEVIs (ASAP2s and VSFP) and one voltage-sensitive dye (Di-4-Anepps) [23,24].Both GEVIs were congenitally expressed in cortical pyramidal neurons in transgenic mice, thus eliminating variables commonly associated with the quality of the adeno-associated virus (AAV) vectors or quality of intracranial injections.

Data Analysis
Optical traces were conditioned and analyzed in Neuroplex (RedShirtImaging).Bleach correction was performed by subtracting an exponential fit from the optical trace.Temporal averaging (n = 4 sweeps), spatial averaging (21 or 37 pixels), low-pass Gaussian filter with 100 Hz cutoff, and high-pass Tau filter (10) were also conducted.For measurements of optical noise (root mean square, RMS) and signal-to-noise ratio (SNR), the low-pass filter was not used.Optical signal amplitude was measured as fractional change in light intensity (∆F/F).The 4th optical peak, caused by a triplet of synaptic inputs at 83 Hz (Train-2) was used to quantify amplitudes in section 3.3.The 1st optical peak, caused by a triplet of synaptic inputs at 8.3 Hz (Train-1), was used to quantify amplitudes in section 3.7.Decay rates of compound EPSPs (cEPSPs) were measured as fractions of the peak amplitude remaining 90 ms after the occurrence of the cEPSP peak.In this setting, a large fraction remaining 90 ms after the peak indicates slow decay of cEPSP.Lost light due to photobleaching was calculated by measuring the amplitude difference between two time points on the same trace (in mV); the two time points were selected at 125 ms and 2990 ms from the beginning of the optical sweep.Resting fluorescence intensity (RFI) was measured in the same region of interest (ROI) in which the photobleaching and GEVI signal amplitude were measured.RFI is expressed in arbitrary units (a.u.) after adjusting for the illumination intensity (light power at the object plane).Data organization, plotting, and statistical testing using analysis of variance (ANOVA) and unpaired Student's t-tests were done in Excel.

Results
Three strains of transgenic mice were used to prepare acute brain slices for electrophysiology optical imaging experiments.Wild type mice were used for experiments with autofluorescence optical signals and voltage-sensitive dye, Di-4-Anepps.Each brain slice (experimental groups varied in size from 31 to 104 brain slices) was stimulated in an identical manner and simultaneous multisite optical recordings were performed using the same experimental setup (light source, optical filters, dichroic mirror, objective lens, and camera).We present a comprehensive analysis of voltage indicator performances, and we point to both advantages (optical sensitivity, speed of response, afterhyperpolarization, spatial spread of voltage transiens) and disadvantages (signal decay time, photobleaching, autofluorescence optical signal bleed-through) attributed to each indicator.

Expression of ASAP2s in Cerebral Cortex
Crossing of the Thy1-Cre and ASAP2s_LoxP mouse lines achieved strong labeling of all cortical layers, with the exception of the L4 lamina.That is, in the ASAP2s channel (green), we found a darker horizontal band coinciding with cortical layer 4 (Fig. 1).This darker band was not caused by a lower density of cells, as both the nuclear stain, Hoechst (Fig. 1A 1 ), and the glial stain, GFAP (Fig. 1A 2 ), detected normal density of cellular elements (Fig. 1A 2 , no abnormal features = none).Pyramidal neurons comprise the majority of nerve cells in neocortex (~80%).Overlapping dendritic trees appear as a "sea of light" with pyramidal cell bodies showing as "dark holes" (Fig. 1B 1 -B 4 ).This finding applies to both Thy1-ASAP2s (Fig. 1B 1 -B 4 ) and VSFP animals [27].

Interplay between ASAP2s and Label-Free Autofluorescence Metabolic Optical Signals
In brain slices collected from wild type mice, we performed optical recording sessions in the absence of GEVIs or dyes (Fig. 3A 1 ).In Fig. 3A, we marked the beginning of the synaptic stimulation train by a gray dashed vertical line.In 24 out of 27 brain slices tested in this way, we observed synaptically-evoked optical signals in the green optical channel (Fig. 3A 2 , Autofluorescence Signal).The mean amplitude of synaptically-evoked autofluorescence optical signals, measured at the stimulation site (ROI-1) in layer 2/3, was 0.99 ± 0.10 % ∆F/F (mean ± s.e.m., n = 24).In 6 brain slices, following the autofluorescence signal recordings, we applied Di-4-Anepps (Fig. 3A 3 ).Without changing stimulation parameters or visual field, we then recorded synaptically-evoked Di-4-Anepps signals (Fig. 3A 4 ).We used Di-4-Anepps signals in the same brain slice to demonstrate that: [1] the brain slice was alive; [2] synapses were activated; and [3] cortex was depolarized.In these experiments, an average amplitude of synaptically-evoked autofluorescence optical signal before voltage-dye staining was 0.89 ± 0.28 % (n = 6).In the same ROI, the average amplitude of synaptically-evoked Di-4-Anepps optical signal (after staining) was 0.64 ± 0.08 % (n = 6).Autofluorescence optical signals (excitation 480/40 nm) and voltagesensitive dye signals (excitation 540 nm) were of an opposite polarity (Fig. 3A   the synaptic stimulation train (Fig. 3B 4 ), the autofluorescence optical signal was still rising after the end of the stimulus train (Fig. 3B 2 ), eventually achieving its peak (p) several hundred milliseconds after the end of the stimulus train (Fig. 3B 2 , arrow).A slow temporal dynamic of the autofluorescence signal (Fig. 3A 2 ), different from that of the VSD signal (Fig. 3A 4 ), suggests that autofluorescence optical signal were not reporting membrane voltage [28].Furthermore, the lateral propagation of the autofluorescence optical signal along the cortical lamina 2/3 (Fig. 3B 1 ,B 2 ), was notably weaker than the propagation of the corresponding VSD signals in the same brain slice, n = 6 (Fig. 3B 3 ,B 4 ).At 550 µm away from the stimulation site, the autofluorescence optical transients were either undetectable or extremely small (Fig. 3B 1 ,B 2 , ROI-4), while at same distance from the stimulation site, 550 µm (Fig. 3B 3 ,B 4 , ROI-4), the VSD signals were clearly distinguishable (n = 6).
The amplitudes of the autofluorescence optical signals tended to decrease with repetitive measurements, from Trial-1 to Trial-2, for example (compare Fig. 3C 1 , ROI-1 versus Fig. 3C 2 , ROI-1).In the autofluorescence optical imaging, experimental trials (Trial-1 to Trial-3) were separated by 60 sec.Each trial was an average of 4 sweeps, 12-25 sec apart.On average, the autofluorescence signal amplitude in the second trial was 88.4 + 2.2 % (n = 24) of the amplitude measured in the first trial (Fig. 3C 3 ).On average, the signal amplitude in the third trial, Trial-3, was 87.9 + 4.3 % (n = 20) of the amplitude measured in the first trial, Trial-1 (Fig. 3C   moxifen (TAM) diet, from 7 to 2-3 days, we were able to achieve weak expression of GEVI.In weakly expressing ASAP2s mice, we observed slow negative undulations superimposed onto fast voltage signals (n = 4 brain slices, Fig. 3D, black traces).To illustrate this point, we have included the autofluorescence optical signal from Fig. 3C 2 onto the ASAP2s optical signal (Fig. 3D, thin and thick trace).In weakly-expressing ASAP2s mice, the autofluorescence optical signal influenced the ASAP2s optical signal (Fig. 3D).However, in Cux2-ASAP2s mice with strong expression of ASAP2s, the autofluorescence optical signal was eliminated by the strong baseline fluorescence of ASAP2s (resting fluorescence level).In other words, in strongly expressing ASAP2s brain slices, or strongly expressing VSFP brain slices, the autofluorescence optical signal was eclipsed by the strong GEVI signal (present study); or strong expression of a fluorescent calcium sensor [29].This was obvious in the time windows ~400 ms after the end of the stimulation train, in which the negative peak of the autofluorescence optical signal was expected to deviate the ASAP2s optical signal (Fig. 3E).Instead, the slow, negative waves belonging to the autofluorescence optical signals were not seen in these ASAP2s recordings (n = 30 slices).In remote ROIs (Fig. 3E, ROI-4), where autofluorescence optical signal is virtually non-existent (thin trace), the ASAP2s optical signal showed a fast negative transient, akin to afterdepolarization (thick trace).

VSFP, Di-4-Anepps and ASAP2s, Side-by-Side Comparisons
Using an identical experimental paradigm (8.3 Hz and 83 Hz stimulation) and the same recording setup (same light source, objective lens, and camera), we compared the performances of 3 voltage indicators, two GEVIs (VSFP and ASAP2s), and one VSD (Di-4-Anepps).Three synaptic ).In the context of evoked cEPSPs, ASAP2s produced optical signals with the highest SNR.An indicator with less bleaching at a given standard excitation intensity could be illuminated with more intense light while keeping the available usable recording time.More intense light would increase the indicator's SNR [30].One potential approach to address the relation between illumination intensity and SNR would be to normalize the calculated SNR by the bleaching time constant.However, this was not done in the present study.
Interestingly, the decay phases of the cEPSP showed different temporal dynamics between the 3 voltage indicators.We estimated the "rate of decay" by measuring the fraction of the optical signal (peak = 100%) remaining 90 ms after the onset of the extracellular stimulus (Fig. 4E, gray ball).We found that VSD optical signals showed the slowest decay.In the VSD recording mode, at 90 ms post stimulus, the optical signal repolarized down to only 26.16 ± 1.65 % (n = 31) of its peak value.In the VSFP recording mode, the optical signal repolarized down to 18.05 ± 0.71 % (n = 104), while in the ASAP2s experiments the optical signals repolarized near-completely; 5.57 ± 0.8 % (n = 71) for Thy1 promoter and 1.11 ± 1.33 % (n = 37) for the Cux2 promoter (Fig. 4F).An ANOVA was conducted to compare the effect of GEVI on the decay phase of the cEPSP optical signal.Comparisons were made between experimental groups: Di-4-Anepps, VSFP, ASAP2s_Thy1, and ASAP2s_Cux2.There was a significant difference in optical signal decay rate at the p < 0.01 level for the 4 experimental groups, F (3, 239) = 101.78,p = 1.7 × 10 −42 .Post-hoc testing determined that cEPSP decay phase in VSD group was significantly slower than in the VSFP group (p < 0.000001) and the ASAP2s_Thy1 group (p = 7.13 × 10 −23 ), and significantly slower than in the ASAP2s_Cux2 group (p < 0.000001).The cEPSP decay phase in the VSFP group was significantly slower than in either ASAP2s_Thy1 (p < 0.000001) or ASAP2s_Cux2 (p < 0.000001) groups.There was a statistically significant difference between EPSP decay rates in optical recordings using ASAP2s_Thy1 versus ASAP2s_Cux2 (p = 0.0028).Overall, these data indicate that the repolarization phase of an evoked cEPSP was the slowest in the Di-4-Anepps group (Fig. 4).These data may also suggest that optical-local field potentials (LFP) decay-rate is inversely proportional to the number of circuit elements contributing to compound (population) optical signal.In Di-4-Anepps experiments, all membranes were labeled with fluorescent indicator, hence the slowest signal.In ASAP2s_Cux2 experiments, where ASAP2s expression is significantly weaker than in ASAP2s_Thy1 experiments, the fewest membranes were labeled with fluorescent indicator, and hence the fastest EPSP decay rate.The difference in decay time could be attributed to various factors.It's essential to consider the GEVI or VSD's real-time dependence on the membrane potential change, the membrane components of the signal source, and autofluorescence's potential impact on ASAP2s and chi-VSFP.

Temporal Summation
Using the same experimental paradigm (8.3 Hz) and recording setup, we evaluated the temporal summation of synaptically-evoked depolarization in VSFP and ASAP2s experiments.A glass stimulation electrode was positioned in L2/3 and synaptically evoked optical signals were recorded at the stimulation site.We show 5 brain slices from the VSFP experimental groups (Fig. 6A) and 5 brain slices from the ASAP2s group (Fig. 6B).Interestingly, in the VSFP experimental series, the 3rd synaptic event often exhibited a higher amplitude than the 1st synaptic event (Fig. 6A, dashed horizontal line), reminiscent of temporal summation of synaptic potentials (paired pulse facilitation).On the contrary, in the ASAP2s experiments, the 3rd synaptic event often showed a smaller amplitude than the 1st synaptic event in the same optical trace (Fig. 6B  Optical signal amplitudes for the data shown in C3. (C6) Each data point has two coordinates x and y; where "x" is RFI (arbitrary units) and "y" is signal amplitude (∆F/F).Each data point is from a different brain slice.Inset: ASAP2s and VSFP traces on the same scale, amplitude = 1%, time = 50 ms.(D) Brain slice obtained from a VSFP transgenic mouse.Synaptically-evoked cortical depolarizations are first recorded in the green emission mode (d1), and then in the red emission mode (d2).Each trace is an average of 4 trials.Scale, 200 µm.

Apparent Cortical Hyperpolarization
Another difference between VSFP and ASAP2s was the absence or presence of a fast negative voltage transient following the evoked depolarizing voltage transient in cortical layer 2/3.This negative voltage transient is dubbed "apparent hyperpolarization" (Fig. 6B).First, we made sure that we conditioned optical traces in both exper-imental groups with an identical set of low-pass and highpass digital filters.In the VSFP experimental series (n = 104 brain slices, 34 mice), on their return to the baseline (repolarization phase) the optical traces did not cross the baseline established before the arrival of the stimulus (Fig. 6A, baseline is marked by a beige horizontal line).VSFP traces approached the baseline, but never crossed (Fig. 6A, black arrow).Conversely, in the ASAP2s experiments (n = 30 brain slices, 10 animals), optical traces regularly crossed the baseline established before the arrival of the stimulus (Fig. 6B, purple horizontal line).The ASAP2s traces traversed the baseline after the 1st, 2nd, and 3rd synaptic event (Fig. 6B, black arrows).In brain slice experiments, the baseline established before stimulation was reminiscent of a resting membrane potential, and negative deflections in the ASAP2s optical signal (Fig. 6B, black arrows) are reminiscent of cortical hyperpolarizations in population voltage imaging experiments [31,32].Fast hyperpolarizations in ASAP2s traces (Fig. 6B, black arrows) were not caused by underlying autofluorescence optical signals (Fig. 3C 1 ), because weak autofluorescence signals have a slower dynamic (Fig. 3B 2 ), autofluorescence signals are eclipsed by the high background GEVI fluorescence (Fig. 3E, ROI-1), and rapid negative voltage transients can be detected at distances from the stimulation electrode at which autofluorescence signals are non-existent (Fig. 3E, ROI-4).Optical traces displayed in Figs.1,2,3,4,5 have been conditioned by subtracting an exponential function that mimics the exponential decline in RFI, known as "photobleaching".Several factors might cause a gradual shift (decline in optical signal), including heat drift in LED light sources, electron fluctuations in the CCD device over time, and photobleaching of the fluorophore.Next, we will address the bleaching of the two GEVI variants, VSFP and ASAP2s, using the same LED source and CCD camera.

Photobleaching and Dual Emission
The camera used in the current study (Neuro-CCD, RedShirtImaging) converted the incoming photon flux into voltage (current-to-voltage converter).The RFI (in mV) is the fluorescence level at the beginning of an optical recording sweep (e.g., time = 0 ms).In any type of a fluorescence measurement, RFI at the beginning is different from that recorded at the end of an optical sweep (e.g., time = 3000 ms), due to photobleaching.In the current study, the bleaching rate of a fluorescent indicator was quantified by measuring the amount of the RFI reduction (in mV) during a 3-second optical sweep (Fig. 6C 1 -C 3 , lost light).Contrary to other figures, in Fig. 6C 1 ,C 2 , optical traces are displayed with normal polarity.We found that photobleaching in the VSFP recordings was approximately 10-fold slower compared to the photobleaching occurring in the ASAP2s experiments (Fig. 6C 1 ,C 2 ).More specifically, the amount of lost light per 3 seconds in VSFP was 31.1 ± 1.3 mV (n = 28), where as in ASAP2s_Thy1 and ASAP2s_Cux2 experiments, the "lost light" was about 10-fold higher, 476.8 ± 37.99 mV (n = 33) and 505.84 ± 38.26 mV (n = 31), respectively (Fig. 6C 3 ).In VSFP experiments, the RFI measured at the beginning of the optical trace was 5.993 ± 0.568 a.u.(n = 28), which was notably higher than the RFI achieved in ASAP2s_Thy1, 2.011 ± 0.158 a.u.(n = 30).In the ASAP2s_Cux2 experiments, the RFI was low due to weak tamoxifen-induction of the ASAP2s expression, 0.319 ± 0.046 a.u.(n = 31), (Fig. 6C 4 ).Next, using only the data used in panels C 3 and C 4 of Fig. 6, we quantified amplitudes of the first voltage transient (8.3 Hz train).These amplitude measurements were consistent with the amplitude measurements shown in Fig. 4D that were made on a larger experimental set (e.g., n = 104), and using the 4th voltage peak caused by an 83 Hz train (Fig. 4D).Despite a strong bleaching in ASAP2s (Fig. 6C 3 ) and weaker fluorescence levels (Fig. 6C 4 ), the ASAP2s optical signals consistently showed higher optical signal amplitudes (∆F/F) compared to the VSFP signals (Fig. 6C 5 ).In Fig. 6C 6 (inset), VSFP and ASAP2s traces are displayed on the same amplitude scale (1%, ∆F/F).To illustrate the relation between RFI and the optical signal amplitude in the same ROI where the RFI was measured, we plotted an Amplitude vs RFI graph (Fig. 6C 6 ).Each data point is the product of two coordinates: x-axis (RFI), and y-axis (Amplitude).The VSFP group showed strong RFI levels but weak optical signal amplitudes (Fig. 6C 6 , beige data points).The ASAP2s_Cux2 group, in which expression of ASAP2s was induced by a 2 to 7-day tamoxifen diet, showed weak RFI, but a strong optical signal (Fig. 6C 6 , red data points).The ASAP2s_Thy1 group, in which expression of ASAP2s was driven by Thy1 promoter, since the animal's conception, demonstrated stronger RFI levels than the ASAP2s_Cux2 group and strong optical signals (Fig. 6C 6 , purple data points).
While ASAP2s emits only in the green channel, VSFP generates a dual emission in both green and red, stemming from the presence of two fluorophores in each VSFP indicator molecule [33].When excited by 488 nm light, the voltage indicator VSFP glowed in green channel (emission 510-545 nm), but also in the red spectra (emission 578-625 nm) [27].In the same brain slice (Fig. 6D), we recorded synaptically evoked optical signals first in the green (donor) and then in the red emission channel (acceptor).These recordings produced signals of opposite polarity (n = 6 brain slices).Invariably, the signal to noise ratio in the green emission channel (Fig. 6D, green emission) was better than in the red emission channel (n = 24 ROIs in 6 brain slices).The dual emission of VSFP (Fig. 6D) could be useful for in vivo experiments, in settings in which both channels, donor and acceptor, are recorded simultaneously [25].Optical signals in the red channel could potentially be used to correct for mechanical artifacts, as well as for conditioning optical signals.

Signal Polarity
The true polarity of VSFP and ASAP optical signals is shown in Fig. 6C 1 ,C 2 .All three indicators used in the current study (VSFP, ASAP2s, and Di-4-Anepps) decreased light with depolarization (negative optical signal).On the contrary, autofluorescence optical signals increased the in-tensity of emitted light (510 nm) with cortical depolarization (synaptic stimulation).In all figures of the current manuscript, except in Fig. 6C 1 ,C 2 , optical traces are shown with inverted polarity.We feel that in display (presentation), voltage imaging optical signals (VSFP, ASAP2s, and Di-4-Anepps) should increase with depolarization.

Origin of GEVI Physiological Signal
In Figs.3,4,5,6, all optical signals were evoked by synaptic stimulation, involving extracellular current pulse delivered in L2/3.Depolarization responses of many L2/3 pyramidal cells were projected to the same detector pixel, and for that reason the reported optical signals are similar to LFP, which are to be dominated by synaptic potentials [38].Population voltage imaging has a better spatial resolution than the LFP signal [17,38].Electrical LFPs report extracellular current densities.When a depolarization wave passes under an LFP electrode, the LFP signal flips its polarity.In contrast, the GEVI method reports transmembrane voltage changes.In our GEVI recordings, a membrane depolarization is always with positive polarity, and membrane hyperpolarization is always negative, regardless of the voltage transient's propagation velocity or direction.One should also consider the possibility that this negative signal component (apparent hyperpolarization) may represent an activity-related alkalinization of extracellular space to which some GEVIs are sensitive [39,40].

Population GEVI Imaging for Understanding Brain Functions
Integration of fragmented sensory inputs is best studied by measuring neuronal voltages at multiple sites [41,42].In association cortices, integration of primary sensory information with other sensory modalities (touch, light, or sound) and quick comparisons with previously stored patterns (memory), lead to the generation of adequate motor actions.These operations engage both local and distributed region-wide computations [43] conducted using both rapid and slow changes in neuronal membrane voltage [44][45][46].Only macroscopic GEVI voltage imaging (GEVI-produced optical LFP) can be used to achieve 4 cardinal requirements of a modern systems neuroscience: (1) To record fast changing voltage transients (compound action potentials (APs) and EPSPs).
In departure from standard voltage-sensitive dye imaging [4,17], GEVI imaging offers three important advantages: (a) physiological signals restricted to a specific cell population of interest (e.g., neocortical layer 2/3 pyramidal cells); (b) GEVI transgenic mice do not require removal of dura or skull-bone -imaging through a thinned skull [43]; and (c) repeated imaging sessions in living mice over the time course of an experiment lasting days [47].

Autofluorescence Optical Signals
In the absence of any fluorescent labeling (no GEVI expression or dye application), autofluorescence optical signals (excitation 470 nm, emission 510 nm) reliably detect synaptically evoked "events" in cerebral cortex.Amplitudes of autofluorescence optical signals (∆F/F) were comparable to signals obtained with VSD in the same brain slice and ROI, but the time course of an autofluorescence optical signal was notably slower.In the autofluorescence imaging recordings, signal polarity was opposite to the VSD or the GEVI signal polarity.The autofluorescence signal dynamics was slow (signal amplitude continued to grow after the stimulus train has ended), and the signal-tonoise ratio (signal quality) was significantly worse than in the VSD or GEVI recordings (Fig. 3).Our data show that one does not need any indicator to evaluate the viability of a brain slice or determine the success of synaptic stimulations.In slices of poor health, or when stimulation current intensity was 20% of that used in Fig. 3, autofluorescence optical signals were weak or absent.Our experiments indicate that autofluorescence optical signals, which may be due to flavoprotein [48][49][50], only occur in viable brain slices and only with adequate synaptic stimulation.Interestingly, autofluorescence optical signals cannot be used for studying propagation of voltage waves through cortical parenchyma, as these signals decay quickly with distance (Fig. 3B 1 ,B 2 ), compared to VSD or GEVI optical signals, which regularly report cortical voltage transients at 550 µm distance from the stimulation site (Fig. 3B 3 ,B 4 ).

GEVI Optical Signals
Both VSFP and ASAP2s produced optical signals that can be used for comparing amplitudes and time courses (rise time, decay time, and temporal summation) of the evoked voltage waveforms between experimental groups.For example, comparing an experimental group composed of Alzheimer's disease model mice versus an experimen-tal group composed of their healthy littermates [51].Both GEVI indicators, VSFP and ASAP2s, can be used for monitoring "vertical" propagation of evoked depolarizations between cortical layers (e.g., from L4 to L2/3), or "horizontal" propagation along one cortical lamina (e.g., along layer 2/3, Fig. 5).One important difference between the two GEVI indicators was temporal summation at the 120 ms stimulus interval (8.3 Hz).While the VSFP optical signals integrated photons and showed a gradual increase in the synaptic event amplitude at the end of a synaptic train (activity-dependent amplitude facilitation, Fig. 6A), ASAP2s optical signals showed gradual declines (activity-dependent amplitude depression, Fig. 6B).
The half-width (duration at half amplitude) of the cEPSP voltage waveforms in ASAP2s and VSFP experimental series were similar (compare Fig. 4A vs Fig. 4C), suggesting that these two GEVI variants faithfully report cEPSP decay phases in cerebral cortex, while the voltage-sensitive dye Di-4-Anepps alters cortical cEPSP waveforms.Two factors may potentially account for a slightly slower decay phase in the Di-4-Anepps experimental series (Fig. 4B,E,F).First, ASAP2s and VSFP signals were restricted to excitatory pyramidal cells, while Di-4-Anepps indiscriminately labeled all neuronal and nonneuronal membranes.It is not clear how optical signals from interneurons, astrocytes, blood vessels, and other elements affect the cEPSP voltage waveforms.Second, Di-4-Anepps exerts pharmacological effects on neurons [52], therefore the slowing of the EPSP decay phase observed in the present study (Fig. 4B,E,F) may also be an undocumented pharmacological effect of this voltage-sensitive dye.However, to demonstrate a pharmacological effect, one should present the dye-induced effects (e.g., a change in the time course of the voltage transient) with electrophysiological methods, which was not done here.Bayram Yilmaz for supervision of students, and to Kumiko Watanabe and Bugra Ozgun for technical support.

Conclusions
1 -A 4 ,B 1 -B 4 ).Note that optical traces are displayed with inverted polarity.Two types of optical signals, autofluorescence and voltage sensitive dye (VSD), exhibited different temporal dynamics.In Fig. 3B, we marked the end of the synaptic stimulation train by a gray dashed vertical line.While the VSD signal begins to decay immediately at the end of

Fig. 1 .
Fig. 1.Thy1-ASAP2s mice.ASAP2s is expressed in all pyramidal cells of all cortical layers.(A1) A brain section (30 µm) from a Thy1-ASAP2s mouse, stained with nuclear stain, Hoechst (10× lens, composite image).The granular layer is marked by two white lines.(A2) Same brain section -expression of the glial marker GFAP.None -indicates a lack of interesting features in L4. (A3) Expression of ASAP2s in the GFP optical channel.Fluorescence is weak in L4 -dark band.(A4) White lines on the right-hand side delineate cortical layers.w.m., white matter.(B1) A different brain section, nuclear stain (Thy1-ASAP2s animal, layer 2/3, 20× lens).(B2) Neuronal marker NeuN.(B3) Expression of ASAP2s.Because ASAP2s molecules are located in the membrane, and all neurons are labeled, the neuropile is "fused" and the neuronal cell bodies appear as dark holes.(B4) A merge of three channels.Arrows marks two out of several NeuN-negative (non-neuronal) cells in this field of view (FOV).GFAP, glial fibrillary acidic protein; GFP, green fluorescent protein.
3 ).Both the autofluorescence and ASAP2s signals were recorded in the same optical channel (green fluorescent protein, GFP, excitation 480/40 nm), meaning there was a possibility of autofluorescence optical signals interfering (mixing) with the ASAP2s signals.By reducing the number of days that the Cux2-ASAP2s animals were fed the ta-

Fig. 3 .
Fig. 3. Optical signals of various origins evoked by identical stimuli.(A1) Autofluorescence in brain slice from wild-type mice before application of dye.The GFP optical channel filters are described in white font.(A2) Synaptically-evoked optical signals in an unstained slice -GFP channel.Each region of interest (ROI) is a spatial average of 37 pixels.Each trace is temporal average of 4 subsequent sweeps (20 sec inter-sweep interval).Vertical dashed lines mark onsets of the stimulus trains.(A3) Same brain slice, same field of view (FOV) and stimulus, except a voltage sensitive dye, Di-4-Anepps, was injected into the bath.LED and Di-4-Anepps optical filter set described in white font.(A4) Di-4-Anepps optical signals.(B1) Autofluorescence (GFP channel) in brain slice from transgenic Cux2-ASAP2s mice, in which tamoxifen induction was unsuccessful.(B2) Synaptically-evoked autofluorescence optical signals.The peak of this label-free optical signal ("p") occurs >100 ms after the last stimulus pulse in train (vertical dashed line).(B3) same brain slice after application of Di-4-Anepps.(B4) The peak of the Di-4-Anepps optical signal ("p") coincides with the last stimulus pulse in the train.(C1,C2) Two subsequent experimental trials in the same FOV, same stimulus intensity.Amplitude of the autofluorescence optical signal declines in the later trial (Trial-2, lost amplitude).(C3) Changes in the optical signal amplitude across 3 subsequent experimental trials (n = 24 recordings in 22 brain slices).(D) Optical signals obtained in Cux2 mice with a very weak expression of ASAP2s.Negative deflections in the ASAP2s optical signal (black) suggest an underlying autofluorescence optical signal (gray).In this and the following panes, thin black traces are autofluorescence optical signals from C2, superimposed onto the ASAP2s optical signal (thick black trace).(E) Optical signal in brain slice from a Cux2-ASAP2s mouse in which expression of the ASAP2s gene was strong (tamoxifen, TAM diet, 7 days).Rectangles mark time windows in which an autofluorescence optical signal, if present, should inflict a negative deflection.ROI-4 is "too far away" from the stimulation site (ROI-1), for label-free (metabolic) optical signals to reach it, but not for the ASAP2s (voltage) optical signals.All optical signals in this figure are shown with inverted polarity.

Fig. 4 .
Fig. 4. Three voltage indicators.(A) Transgenic mice expressing VSFP in cortical pyramidal neurons.Surface of brain slice with stimulating electrode in L2/3.Stimulation was the same as in Fig. 3. Voltage indicator is VSFP.(B) Same as in A, except the subject was a wild-type mouse (Black 6) and the voltage indicator is voltage-sensitive dye, Di-4-Anepps.(C) Same as in A, except mouse was transgenic, expressing voltage indicator ASAP2s in pyramidal neurons (Thy1).(D) Signal amplitude at the stimulation site ROI 1. Four experimental groups based on the voltage indicator used.Each dot represents one measurement in one brain slice.VSFP n = 104, VSD n = 31, ASAP-Cux2 n = 37, ASAP-Thy1 n = 71.The ASAP2s signal amplitude was not affected by the type of transgenic mouse, Thy1-Cre or Cux2-CreER (no significant difference).(E) The signal decay dynamics was quantified at 90 ms after the stimulus pulse; and expressed as a percentage of the first peak amplitude.(F) Quantifications of the signal decay rate (explained in E) are plotted here as raster and bar graphs, where "bar" indicates mean ± standard error of mean, SEM.*** p < 0.0001; # p > 0.05.Same number of experiments (n) as in panel D. GEVI, genetically encoded voltage indicator; VSFP, GEVI made by the T. Knopfel laboratory; VSD, voltage sensitive dye.

Fig. 5 .
Fig. 5. Spatial spread of synaptically evoked voltage transients assessed by three voltage indicators.(A1) Transgenic mice expressing VSFP in cortical pyramidal neurons.Surface of brain slice with stimulating electrode in L2/3.A triplet of stimulus pulses was delivered at 120 ms interval (8.3 Hz).(A2) Synaptically evoked cortical depolarizations were imaged in five ROIs simultaneously (ROI 1-5).(A3) Signal amplitude at each ROI is normalized by the signal amplitude obtained at the stimulation site ROI 1.Each bar is a mean ± sem of 20 measurements (20 brain slices, from 12 mice).(B1-B3) Same as in A, except this animal is a wild type, and voltage indicator is Di-4-Anepps (n = 20 brain slices, 9 mice).(C1-C3) Same as in A, except mouse is transgenic, expressing voltage indicator ASAP2s in pyramidal neurons (Thy1 promoter) (n = 20 brain slices, 7 mice).(A3,B3,C3) Dashed line marks the best fit (linear), with R value displayed.The negative slope of the distance-dependent amplitude decay was slightly steeper for the VSFP data (-0.231)compared to the Di-4 data (-0.208), or the ASAP2s data (-0.216).The normalized ROI-5 amplitude was compared between three experimental groups: VSFP, Di-4 and ASAP (unpaired t-test).VSFP produced significantly smaller amplitudes at ROI-5 than the other two indicators did (*, p < 0.05).
, dashed horizontal line), reminiscent of synaptic depression or synaptic fatigue.Note that both VSFP and ASAP2s signals were recorded in the same optical channel in which we detect autofluorescence optical signals (GFP, excitation = 470 nm, emission = 510 nm).However, VSFP signals show facilitation (Fig. 6A) while the ASAP2s signals show depression, on the same experimental paradigm.

Fig. 6 .
Fig. 6.Temporal dynamics of the two genetically encoded voltage indicator (GEVI) variants, VSFP and ASAP.(A) Three synaptic pulses were delivered into L2/3 at 120 ms interval (8.3 Hz).Five brain slices expressing VSFP -one trace per slice.Red arrow indicates that the 3rd peak is of a greater amplitude than the 1st peak.Black arrows emphasize that membrane voltage is always above the resting membrane potential (beige horizontal line).Each trace is an average of 12 trials.(B) Identical stimulus as in A. Five traces from 5 brain slices expressing ASAP2s.Red arrow indicates that the 3rd peak is of a lower amplitude than the 1st peak.Black arrow indicates voltages more negative than the resting potential.Each trace is an average of 3 trials.Note different amplitude scales in A versus B. (C1) Five recordings from 5 brain slices in the VSFP group are shown with normal polarity and without a correction for bleaching.(C2) Same as in C1 except, the mouse is ASAP2s.Note a 5-fold difference in the mV scales.(C3) The average amount of lost light (in mV, per 3 second of light exposure) is plotted for 3 experimental groups.(C4) Resting fluorescence intensity (RFI) for 3 groups analyzed in C3. (C5) All three voltage indicators evaluated in the present study (ASAP2s, VSFP, and Di-4-Anepps) have demonstrated versatility in population voltage imaging of synaptically-evoked cortical depolarizations, in vitro, in brain slices.Transgenic mouse lines carrying VSFP or ASAP2 offer major practical and conceptual advantages.Transgenic expression eliminates invasive and laborintensive intracranial injections of AAV vectors.A fast OFF dynamic of the GEVI variant, ASAP2s, renders this indicator the first choice for studying rapidly changing voltage fields in cerebral cortex (e.g., cortical oscillations).A strong resting fluorescence of VSFP, renders this indicator the first choice for intact-skull imaging (imaging through a thinned skull bone).Among three indicators tested, ASAP2s was the only one showing apparent cortical hyperpolarizations.In the same optical channel in which we recorded ASAP2s optical signals (excitation 470 nm, emission 510 nm), we also recorded autofluorescence optical signals.These "negative" optical signals, autofluorescence optical signals, should not be confused for hyperpolarizing cortical events in ASAP2s traces.Autofluorescence optical signals have an opposite polarity and a slow dynamic compared to ASAP2s signals of evoked cEPSPs.When ASAP2s expression is low, like in Cux2_ASAP2s mice, the autofluorescence optical signals mix with the ASAP2s signals, and introduce slow wave artifacts.ASAP2s bleaches notably faster than VSFP.While VSFP produces optical signals in both green and red channel simultaneously, ASAP2s has no functional signal and no fluorescence in the red segment of the visible light.ASAP2s has approximately 7-fold greater signal amplitude (∆F/F) than VSFP and 3-fold greater signal amplitude than Di-4-Anepps.