Sepsis-associated encephalopathy is a common brain diseases, presenting severe
diffuse brain dysfunction. The umbilical cord mesenchymal stem cells have been
reported to have protective role for treating diseases, while its role in
sepsis-associated encephalopathy remained elusive. This brief report investigated
the therapeutic effect of umbilical cord mesenchymal stem cells on
sepsis-associated encephalopathy in mice model and uncovering the underlying
mechanism. The sepsis-associated encephalopathy mice were injected with 3 mg/kg
lipopolysaccharide. An enzyme-linked immunosorbent assay was carried out to
determine the production of inflammatory cytokines. Morris water maze test was
used to evaluate mice’s neurological dysfunction. Cell apoptosis and tissue
injury of the cerebral cortex were assessed using terminal deoxynucleotidyl
transferase-mediated dUTP nick end labeling (TUNEL) assay and HE staining. Evans
Blue leakage detection was used to examine the blood-brain barrier integrity. The
protein levels were determined using Western blot. Results showed that
the productions of inflammatory cytokines including interleukin 6 (IL-6),
interleukin-1
Cite this article
105
Downloads
4
Citations
371
Views
Announcements
Open Access
Communication
Therapeutic effects of human umbilical cord mesenchymal stem cell on sepsis-associated encephalopathy in mice by regulating PI3K/AKT pathway
Zhe Zhang1, Li Wang2, Feng Li1, Xiangfeng Qian1, Zhixing Hong1, Longchuan Wu1, Yinsheng Jiang1, Haiqiang Hu3,4,*
Show Less
1
Department of Emergency Medicine, The First People's Hospital of Yuhang District Hangzhou, 311100 Hangzhou, Zhejiang, China
2
Department of Emergency Medicine, The Second Affiliated Hospital Zhejiang University School of Medicine, 310002 Hangzhou, Zhejiang, China
3
Department of Cardiovascular Medicine, The First People's Hospital of Yuhang District Hangzhou, 311100 Hangzhou, Zhejiang, China
4
Department of Cardiovascular Medicine, The Second Clinical Medical College of Zhejiang Chinese Medicine University, 310053 Hangzhou, Zhejiang, China
*Correspondence: haiq_hu@163.com (Haiqiang Hu)
J. Integr. Neurosci. 2022, 21(1), 38;
https://doi.org/10.31083/j.jin2101038
Submitted: 3 June 2021 | Revised: 5 August 2021 | Accepted: 15 September 2021 | Published: 28 January 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract
Keywords
Sepsis-associated encephalopathy
Umbilical cord mesenchymal stem cells
Cognitive dysfunction
Cerebral cortex
PI3K/AKT pathway
Figures
Fig. 1.