IMR Press / JIN / Volume 20 / Issue 4 / DOI: 10.31083/j.jin2004087
Open Access Original Research
Network pharmacology and the experimental findings of Bushenhuoxue formula for improving hippocampal neuron injury in vascular demented rats
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1 The Graduate College, Hebei University of Chinese Medicine, 050091 Shijiazhuang, Hebei, China
2 The Basic Medicine College, Hebei University of Chinese Medicine, 050200 Shijiazhuang, Hebei, China
3 The Acupuncture and Massage College, Hebei University of Chinese Medicine, 050200 Shijiazhuang, Hebei, China
4 Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease, 050091 Shijiazhuang, Hebei, China

These authors contributed equally.

J. Integr. Neurosci. 2021 , 20(4), 847–859; https://doi.org/10.31083/j.jin2004087
Submitted: 29 July 2021 | Revised: 26 October 2021 | Accepted: 3 November 2021 | Published: 30 December 2021
Copyright: © 2021 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license (https://creativecommons.org/licenses/by/4.0/).
Abstract

We used network pharmacology to predict the correlation between the pathway of Bushenhuoxue formula in the treatment of vascular dementia and carried out experiments to verify the correlation between drug composition and disease. By screening the active components and key targets through various databases and drawing the topological network diagram, we obtained 502 effective compound targets, 601 disease targets, 95 disease-related compound targets, and 162 pathways. The pathway related to vascular dementia may be neurodegeneration-multiple diseases, PI3K-Akt signaling pathway, Mitogen-activated protein kinase signaling pathway, or HIF-1 signaling pathway. By detecting the learning and memory ability of vascular dementia rats, the morphology of the hippocampus under the electron microscope, the degree of neuronal damage, and autophagy-related proteins, the results showed that the Bushenhuoxue formula could improve the neuronal injury induced by ischemia in the hippocampus, down-regulate the level of autophagy, and thereby improve learning and memory. Therefore, the Bushenhuoxue formula may improve the ischemic injury of neurons by regulating the mechanism of neuronal autophagy.

Keywords
Autophagy
Bushenhuoxue formula
Network pharmacology
Vascular dementia
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