IMR Press / JIN / Volume 19 / Issue 1 / DOI: 10.31083/j.jin.2020.01.1248
Open Access Original Research
NF-κB “decoy” inhibits COX-2 expression in epileptic rat brain
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1 Functional Laboratory, Dalian Medical University, Dalian 116044, P. R. China
2 Department of Neurosurgery, The First Affiliated Hospital of Dalian Medical University, Dalian 116044, P. R. China
3 National-Local Joint Engineering Research Center for Drug-Research and Development (R & D) of Neurodegenerative Diseases, Dalian Medical University, Dalian 116044, P. R. China
*Correspondence: zhaoj@dmu.edu.cn (Jie Zhao)
J. Integr. Neurosci. 2020, 19(1), 77–87; https://doi.org/10.31083/j.jin.2020.01.1248
Submitted: 25 November 2019 | Accepted: 16 March 2020 | Published: 30 March 2020
Copyright: © 2020 Xu et al. Published by IMR press.
This is an open access article under the CC BY-NC 4.0 license (https://creativecommons.org/licenses/by-nc/4.0/).
Abstract

There is a need to investigate the role of nuclear factor kappa B in the regulation of cyclooxygenase-2 expression in the epileptic rat brain and cultured hippocampal neurons. Immunofluorescence and polymerase chain reaction was used to detect the expression of nuclear factor kappa B and cyclooxygenase-2. In cultured hippocampal neurons and rat brain: the control group compared with the normal group, nuclear factor kappa B expression in the hippocampal dentate gyrus, cerebral cortex, the piriform cortex brain regions were significantly increased (P < 0.01). This is accompanied by a significant increase in cyclooxygenase-2 protein and mRNA expressions in the hippocampus (P < 0.01). In the experimental group compared to the control group, the nuclear factor-kappa B expression in the hippocampal dentate gyrus, cerebral cortex, piriform cortex, and other brain regions was significantly lower (P < 0.01), with the accompanying decrease in cyclooxygenase-2 protein and mRNA expression (P < 0.01) in the hippocampus. In conclusion, κB-decoy can inhibit nuclear factor kappa B activation in epileptic rat brain and cyclooxygenase-2 overexpression.

Keywords
NF-κB
κB-decoy
cyclooxygenase-2
seizures
Figures
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