IMR Press / FBL / Volume 9 / Issue 6 / DOI: 10.2741/1472

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Human cell proteins and human immunodeficiency virus DNA integration
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1 Department of Pathology, Harvard Medical School, Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts, 02115
2 Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Department of Cancer Cell Biology, Dana-Farber Cancer Institute, 44 Binney Street, Boston, Massachusetts, 02115
Front. Biosci. (Landmark Ed) 2004, 9(6), 3187–3208;
Published: 1 September 2004

Integration, catalyzed by the viral integrase (IN) protein, is a crucial step in the life cycle of all retroviruses including human immunodeficiency virus type 1 (HIV-1). Although purified HIV-1 IN protein is sufficient to catalyze the DNA breakage and joining steps of integration in the absence of any other protein factor, a number of studies indicate that cellular proteins participate in the integration process in cells. These host cell proteins have been proposed to act through binding the pre-integrated viral cDNA substrate, by directly interacting with the IN protein, and/or by repairing the single-stranded DNA gaps that occur at viral/chromosomal DNA junctions during integration. In this paper we summarize the identification and potential roles of specific cell factors in HIV-1 integration. We also present experimental results of human cell proteins that coimmunoprecipitated with HIV-1 IN following its expression in HeLa cells and discuss these results in light of the previously-identified integration cofactors.

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