IMR Press / FBL / Volume 8 / Issue 4 / DOI: 10.2741/1035

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Gene expression in the rhesus monkey endometrium: differential display and laser capture microdissection
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1 Department of OB/GYN, University of Massachusetts Medical School, Worcester, MA 01655, USA
2 Department of Physiology, University of Massachusetts Medical School, Worcester, MA 01655
3 Division of Endocrinology, University of Massachusetts Medical School, Worcester, MA 01655

Academic Editor: William Okulicz

Front. Biosci. (Landmark Ed) 2003, 8(4), 551–558;
Published: 1 May 2003
(This article belongs to the Special Issue Progesterone action and the endometrium)

The primate endometrium is a complex heterogeneous tissue that requires proper maturation to achieve a hospitable environment for implantation. Endometrial differentiation and maturation is primarily controlled through the action of progesterone during the secretory phase. Many of the genes and gene networks that are involved in this process are likely to be induced or inhibited in a temporal, spatial, and cell-type specific context within the endometrium. We have used several approaches to address these latter issues in the rhesus monkey endometrium. The use of differential display with hormonally distinct endometrial cDNA populations prepared from artificially controlled menstrual cycles has allowed us to identify different P-dependent mRNA regulatory patterns during simulated secretory phases. In addition, we have coupled differential display with laser capture microdissection to further study region and cell-type specific changes in the primate endometrium.

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