3D-computer based reconstructions of apoptotic nuclei

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Article

J. Peychl¹, J. Husak¹, H. Spring², M. Cervinka¹, E. Rudolf^1,*

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¹ Department of Medical Biology and Genetics, Charles University Faculty of Medicine in Hradec Kralove, Simkova 870, 500 01 Hradec Kralove, Czech Republic

² Biomedical Structure Analysis Group, DKFZ, Im Neuenheimer Feld 280 (A0601), D-69120 Heidelberg, Germany

Front. Biosci. (Landmark Ed) 2002, 7(6), 8–9; https://doi.org/10.2741/A931

Published: 1 January 2002

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Abstract

We present a 3D-model of apoptotic nuclei of HL-60 cells treated with 10 μg/ml Etoposide (topoisomerase II inhibitor) for 24 hours. The static model was generated from a series of optical sections obtained through a confocal microscope by freeware and shareware graphical programs available in the Internet. Its animation was done by 3D Studio Max. We demonstrate the appearance of typical fragmentation and condensation of chromatin accompanied by its aggregation to the inner side of the nuclear membrane.

Keywords

Apoptosis

Nucleus

In vitro

3-D reconstruction

Computer-assisted

Image processing

Microscopy

Confocal microscopy

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