IMR Press / FBL / Volume 7 / Issue 4 / DOI: 10.2741/devine

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

In vitro ovarian tissue and organ culture: a review

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1 Department of Physiology, The University of Arizona, 1501 N. Campbell Ave P.O. Box 245051, Tucson AZ 85724-5051, USA
Academic Editor:Jodi Flaws
Front. Biosci. (Landmark Ed) 2002, 7(4), 1979–1989;
Published: 1 September 2002
(This article belongs to the Special Issue Molecular basis of ovarian development and function)

Many investigations have utilized techniques for culturing ovarian tissue or isolated follicles in vitro. Whole ovaries from fetal or neonatal rodents have been incubated in organ culture systems. This has been utilized to understand the sequence of follicle formation and its hormonal requirements, activation of quiescent follicles, follicular growth and development, and acquisition of steroidogenic capabilities. Adaptations of this technique include incubation of ovaries in a chamber continuously perfused with medium or perfusion of medium through the intact vasculature. Late follicular development, ovulation, and steroidogenesis can also be examined in these systems. Another approach has been to culture individual follicles isolated by enzymatic or mechanical dissociation. The majority of this research has focused on improving follicular development in vitro. This review will discuss these various culture techniques and some of the results that have been acquired. Recent results from toxicological studies utilizing whole ovarian cultures performed will also be described. Future applications for ovarian and follicular cultures may include in vitro follicular development for eventual production of offspring from frozen ovarian tissue, mechanistic studies related to the impact of endocrine disruptors and ovotoxicants on ovarian function, and further investigations into follicle activation and development.

Pre-antral Follicle
In vitro
4-Vinylcyclohexene Diepoxide
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