IMR Press / FBL / Volume 5 / Issue 1 / DOI: 10.2741/A488

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
UV-ozone ashing of cells and tissues for spatially resolved trace element analysis
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1 Institut de Physique Appliquée, Ecole Polytechnique Fédérale, PH-Ecublens, CH-1015 Lausanne, Switzerland
2 Synchrotron Radiation Center, University of Wisconsin - Madison, Stoughton WI 53589, USA
3 Istituto di Neurobiologia del Consiglio Nazionale delle Ricerche, Viale Marx 15, 00137 Roma, Italy
4 Paul Scherrer Institut, CH-5232 Villigen PSI, Switzerland
5 Istituto di Struttura della Materia del CNR, Via Fosso del Cavaliere, Roma, Italy
6 University of Wisconsin - Madison, Department of Physics, Madison WI 53706, USA
Academic Editor:Van Meir
Front. Biosci. (Landmark Ed) 2000, 5(1), 10–17; https://doi.org/10.2741/A488
Published: 1 May 2000
(This article belongs to the Special Issue Encyclopedia of neuro-oncology)
Abstract

UV/ozone ashing of thin tissue sections and cell cultures is a simple technique to enhance relative elemental concentrations, while maintaining their spatial location at the sub-micron level. This approach may enhance the capability of spatially resolved analysis techniques to detect the distribution of trace elements in biological matrices. We present results from light microscopy and x-ray spectromicroscopy studies of tissues and cells demonstrating that the micro-structure is very well conserved. We show the signal enhancement resulting from the removal of carbon, which allows otherwise undetectable gadolinium to be mapped in cancer tissue for a novel neutron capture therapy.

Keywords
Ashing
Microchemical Analysis
Trace Element Detection
Spectromicroscopy
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