IMR Press / FBL / Volume 4 / Issue 4 / DOI: 10.2741/A436

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Molecular surface sequence analysis of several E. coli enzymes and implications for existence of casein kinase-2 bacterial predecessor

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1 Laboratory Of Kinetic And Catalysis, Chair Of Physical Chemistry, Chemical Department of Moscow State University, Moscow, 119899, Russia
Front. Biosci. (Landmark Ed) 1999, 4(4), 394–407; https://doi.org/10.2741/A436
Published: 1 April 1999
Abstract

Casein kinase-2 (CK2) is known as pleiotropic eukaryotic protein kinase that phosphorylates significant number of cellular proteins. Not all functions of the protein were registered up to the present time. However, it is known that this Ser/Thr-specific kinase is involved in the cell cycle progression and is essentially required for the eukaryotic cell viability. Fully automated molecular surface analysis procedure for identification of functionally significant surface residues and sequences on the base of protein spatial structure was elaborated. Using the elaborated procedure, several E. coli enzymes spatial structures and sequences were investigated. It was found that most of the casein kinase 2 potential sites found in sequences of enzymes are accessible for modification. Four of the 5 structures studied have CK2 consensus sites that may definitely influence the activity of the enzyme upon phosphorylation. Some of the potential "CK2-sites" has amino acid contents characteristic for physiological substrates of casein kinase 2 in eukaryotes. The main point of the elaborated method and the structural evidence for existence of a putative casein kinase E. coli predecessor or a protein with similar kinase activity are discussed. Physiological, biochemical, structural and evolutionary aspects of the existence of the putative predecessor are considered.

Keywords
Casein kinase 2
Escherichia Coli
Protein structure analysis
Molecular surface analysis
Alkaline phosphatase
Adenylate kinase
Phosphofructokinase-1
Dglyceraldehyde-3-phosphate dehydrogenase
Isocitrate dehydrogenase
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