- Academic Editor
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Background: Streptococcus mutans is a major component of
dental plaque, contributing to cariogenic biofilm formation and inducing dental
caries. Attempts have recently been made to use postbiotic mediators (PMs) to
prevent dental caries. This research evaluated the antimicrobial/antibiofilm
activity of PMs derived from Lactobacillus rhamnosus GG (LGG) and
Lactobacillus reuteri (LR) against S. mutans in vitro.
Methods: PMs were obtained from the Lactobacilli supernatants.
The minimum inhibitory concentration, minimum bactericidal concentration,
antibiofilm potential, and metabolic activity of PMs against S. mutans
were evaluated using CFU/mL, scanning electron microscopy, and XTT
(2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide)
reduction assay. The expression of gtfB gene as one of the most
important genes involved in S. mutans biofilm formation was also
measured using qRT-PCR. Results: CFU score was reduced by both PMs, but
the reduction was only significant in LGG (p = 0.02). Both PMs caused a
significant decrease in the metabolic activity of S. mutans compared
with the controls (p