Fatty Acid Metabolism: A New Perspective in Breast Cancer Precision Therapy

Breast cancer has a special tumor microenvironment compared to other solid tumors, which is usually surrounded by a large number of adipocytes that can produce and secrete fatty acids and adipokines. Adipocytes have a remodeling effect on breast cancer lipid metabolism, while fatty acids and lipid droplets can make breast cancer cells more aggressive. Lipid metabolism, especially the synthesis of fatty acids, is an important cellular process for membrane biosynthesis, energy storage, and signal molecule production. Therefore, blocking the lipid supply to cancer cells or changing the lipid composition has an important impact on the signal transmission and cell proliferation of cancer cells. Alterations in lipid availability can also affect cancer cell migration, induction of angiogenesis, metabolic symbiosis, evasion of immune surveillance, and cancer drug resistance. Fatty acid synthesis and metabolism have received extensive attention as potential targets for cancer therapy, and studies on modulating the tumor lipid microenvironment to improve the sensitivity of antitumor drugs have also been discussed; however, strategies to target these processes have not been translated into clinical practice.


Background
According to Globocan 2020, breast cancer has become the number one incidence of malignancy and now has the fifth highest mortality rate in the world [1].Breast cancer has a special tumor microenvironment compared to other solid tumors, usually surrounded by a large number of adipocytes that can produce and secrete fatty acids (FAs) and adipokines.Adipocytes have a remodeling effect on breast cancer lipid metabolism, while fatty acids and lipid droplets can make breast cancer cells more aggressive [2].
Fatty acids are a type of lipid with rich and diverse biological functions, acting as both energy storage substances and signaling molecules, as well as key components of the cell membrane structure [3].An excess of lipids can lead to the onset of metabolism-related diseases, such as obesity, and also promote tumor development, treatment response, and metastasis [4].Metastasis of tumor cells is a complex process that can be broadly divided into different stages, while cancer cells need to regulate their lipidrelated metabolic pathways and trophic structures to adapt to metastasis during different stages.These adaptations comprise altering the composition of lipid membranes to adapt to other microenvironments, altering lipid-associated signaling substances to overcome the mechanisms of cell death, and facilitating lipid catabolism and anabolism to meet energy requirements and oxidative stress protection [5][6][7].Cancer cells can also use lipid metabolism to regulate stromal cell status and immune cell activity, establish tumor cells and the microenvironment [8], develop therapeutic resistance, and promote cancer recurrence.Thus, lipid metabolism, especially fatty acid metabolism, is an important cell proliferation process.Altered lipid metabolism is an important phenotype of metabolism in cancer cells.Therefore, blocking lipid supply in cancer cells has significant effects on the signal transmission and cell proliferation of cancer cells.In addition, alterations in lipid availability can also affect the tumor microenvironment, thereby influencing cancer cell migration, neovascularization, evasion of immune surveillance, and induction of anticancer drug resistance.Drugs targeting the metabolism of FAs have emerged as a potential therapy for cancer treatment, although therapeutic strategies targeting these metabolic abnormalities have not yet been translated into clinical practice.

Specificity of Fatty Acids Metabolism in Breast Cancer
Due to the large presence of adipocytes in breast tissue, breast cancer is closely related to the biological function of fatty acid metabolism.Fatty acid metabolism includes multiple pathways, such as fatty acid transport, storage in lipid droplets as triglycerides and cholesteryl esters, mobilization from phospholipids and triglycerides, and fatty acid oxidation.In most human tissues, cellular requirements for fatty acids are generally met through dietary intake, whereas the synthesis of fatty acids from de novo is unimportant, except for in the liver and mammary gland.The differences in the importance of fatty acid synthesis methods in normal versus cancerous tissues make it an attractive therapeutic target [9].Dysregulation of fatty acid metabolism is thought to play an important role in the malignant transformation of many different cancers, including breast cancer.Key metabolic enzymes involved in fatty acid synthesis and oxidation play a critical role in the proliferation, metastasis, and invasion of breast cancer cells.
There are two types of mesenchymal cells most associated with fatty acid metabolism in breast cancer: Cancerassociated adipocytes (CAAs) and cancer-associated fibroblasts (CAFs).Once CAAs are activated by cancer cells, they secrete higher levels of proinflammatory cytokines, which induce triglycerides to release more fatty acids and metabolic reprogramming that can promote cancer progression [10].CAFs promote the upregulation of fatty acid transport protein 1 (FATP1) in triple-negative breast cancer cells and contribute to increased uptake of FAs by the tumor microenvironment, which has been shown to promote cancer cell proliferation [11].Lipid metabolites secreted by cancer-associated fibroblasts can also be taken up by tumor cells to increase tumor cell migration, whereas the knockdown of the fatty acid synthase (FASN) in CAFs, or the use of CD36 antibodies can reduce tumor cell migration [12].
Therefore, the exploration of fatty acids and their metabolism, signaling pathways, and effects on tumors and surrounding cells is of great importance in breast cancer.

Non-Essential Fatty Acids
Non-essential fatty acids constitute the majority of FAs in our body and are usually classified into saturated FAs and unsaturated FAs based on their saturation.FA biosynthesis occurs in the cytoplasm of hepatocytes, mammary cells, adipocytes, etc., and is mainly catalyzed by FASN, which catalyzes the conversion of acetyl coenzyme A to palmitate.FASN overexpression and overactivation are often associated with malignant phenotypes and cancer progression [13].Further, palmitic acid can be synthesized as stearic acid and longer FAs by FA elongase, while overexpression of FA elongase is also associated with cancer progression [14].Synthetic fatty acids can be catabolized for energy production via the β-oxidation pathway, which not only provides energy to cancer cells but also generates the energy needed to counteract oxidative stress during tumor metastasis [15].
Different degrees of unsaturation can confer very different biological properties on FAs.For example, palmitic acid has a greater impact on tumor development, is proinflammatory and lipotoxic, and can lead to endoplasmic reticulum stress and an increased induction of ROS.Cellular adaptation to obesity is governed by palmitic acid and leads to an enhanced tumor formation capacity by HRnegative breast cancer cells, through increased chromatin occupancy of the transcription factor CCAAT/enhancerbinding protein beta (C/EBPB) and key downstream regulators, including CLDN1 and LCN2 [16].Conversely, oleic acid, which is mostly stored as triacylglycerol in lipid droplets, is less toxic and has an anti-inflammatory ability and a lower binding affinity for the FA transporter protein CD36-a scavenger receptor in cells that initiate metastasis [17].However, in some studies, oleic acid induces migration through an EGFR and AKT-dependent pathway in breast cancer cells [18].Due to the complexity of the organism, different mechanisms by the same fatty acid also require further clarification in future studies.In addition, the FA component of lipid membranes makes them mobile: regions containing short or unsaturated lipids are more mobile, whereas regions filled with tightly packed saturated fatty acids are less mobile.The saturated fatty acid regions allow different proteins to co-localize, and thus, serve as transduction hubs for cellular signals.Highly migratory cells tend to show increased fluidity in lipid membranes, which facilitates the epithelial-mesenchymal transition in cancer cells and their separation from the vasculature and beyond [19].Conversely, cancer cells with higher membrane rigidity are not sensitive to drug treatment, and oxidative stress induced by chemotherapy drugs or iron-based mechanisms cannot cause damage to cancer cells.In conclusion, differences in the nature and content of these fatty acids influence the effect on cancer cells.

Essential Fatty Acids
Essential fatty acids refer to fatty acids that are indispensable in maintaining body function but cannot be synthesized by the body; thus, must be obtained through the diet (Fig. 1), such as α-linolenic acid (ALA, an omega-3 fatty acid) and linoleic acid (an omega-6 fatty acid), which are polyunsaturated fatty acids (PUFAs) that can be used as signaling molecules.As prerequisite molecules, they generate others: ω-3 and ω-6 PUFA; these derived PUFA can also participate in cell signal transduction or become part of the cell membrane.The effects of these two PUFA are not consistent since the omega-3 PUFA has an antiinflammatory effect, while the omega-6 PUFA is a precursor of proinflammatory molecules related to cancer development [20].Furthermore, the reduction in cancer risk associated with the intake of omega-3 fatty acids may not be limited to their anti-inflammatory capacity, but also to their ability to modulate the expression of genes related to controlling lipid metabolisms, such as sterol-regulatory element binding protein 1 (SREBP1) [21] and peroxisome proliferators-activated receptor α (PPAR-α) [22].Modifying the intake of PUFAs in the diet has been associated with cancer prevention and inhibition of tumor metastasis (Table 1, Ref. [16,18,[20][21][22]).For example, in breast cancer animal models, an ALA-rich diet can increase T lymphocyte infiltration and reduce estrogen receptor α expression, thereby inhibiting tumor growth and metastasis [23].Increasing omega-3 fatty acid intake and decreasing omega-6 fatty acids can serve to reduce the risk of breast cancer in the population at high risk of breast cancer [24].Docosahex- aenoic acid, as an omega-3 fatty acid, can also enhance the antitumor activity of chemotherapeutics [25].Therefore, to establish an anticancer strategy based on dietary intervention using essential FAs, we need to carefully evaluate and optimize the ratio of omega-3 to omega-6 in the diet.

Fatty Acid Synthesis
The biological traits and energy metabolism mechanisms in tumor cells are different than in normal cells.It has been found that fatty acids required for the growth and proliferation of malignant tumors are mainly derived from de novo initio synthetic pathways (Fig. 1), and important enzymes related to fatty acid synthesis and oxidative metabolism are overexpressed in tumor tissues, although are not expressed or under-expressed in normal tissues [26].Overexpression or enhanced activity of ATP citrate lyase (ACLY), an important enzyme in lipid formation related to glucose metabolism, is associated with tumor progression [27].Decreased ACLY expression limits tumor cell proliferation and survival and inhibits tumor cell invasion and metastasis [28].The fatty acid rate-limiting enzyme acetyl-CoA carboxylase alpha (ACACA) is highly expressed in breast cancer, and ACACA depletion reduces fatty acid synthesis and induces apoptosis in breast tumor cells, although not in non-malignant cells [29].Fatty acid synthase (FASN) is another key enzyme in regulating the de novo synthesis of fatty acids.FASN expression is necessary for lipid synthesis and maintenance of fatty acid metabolism levels, especially in the setting of nutritional abnormalities lacking exogenous lipid intake.It has been shown that FASN is required for breast tumor growth and may mediate the occurrence of brain metastases, which can be used as a potential therapeutic target for breast cancer brain metastases [30].Stearoyl-CoA desaturase (SCD) is a membrane protein of the endoplasmic reticulum (ER) that catalyzes the formation of monounsaturated fatty acids from saturated fatty acids.SCD is overexpressed in cancer cells and controls breast cancer cell proliferation by inducing apoptosis and cell cycle arrest, and preventing migration, although the antitumor effects of SCD inhibitors can be reversed by exogenous oleic acid [31].SREBP1, an important transcription factor that regulates fatty acid synthesis, is overexpressed in breast cancer and is associated with reduced survival in breast cancer patients [21].SREBP1 was demonstrated to be significantly associated with epithelialmesenchymal transition and promotes breast cancer growth and metastasis [32].

Fatty Acid Oxidation
Fatty acid β-oxidation (FAO) is a fatty acid modification process through which long-chain fatty acids can be converted into fatty acids of appropriate length for the body's metabolic needs.Acyl-CoA oxidase 2 (ACOX2) is an important rate-limiting enzyme in cellular peroxisomes and is responsible for catalyzing the β-oxidation of branched and long-chain fatty acids.In ER-positive breast cancer cell lines, the expression and translation of ACOX2-i9 are regulated by estrogen, and the knockdown of this enzyme leads to decreased cell viability [33].The JAK/STAT3 signaling pathway is an important pathway in the regulation of lipid metabolism, whereby the inhibition of JAK/STAT3 can prevent the expression of various lipid metabolism genes, including carnitine palmitoyltransferase 1B (CPT1B), which encodes the critical enzyme for FAO.The STAT3-CPT1B-FAO pathway helps breast cancer cells transform into cancer stem cells and initiate chemoresistance; thus, blocking FAO can make cancer cells re-sensitive to chemotherapy, and inhibit stem cell formation in mouse breast tumors in vivo [15].

Fatty Acid Uptake
The application of exogenous fatty acid uptake requires crossing cell membranes, and among the proteins involved in the uptake of FAs, CD36 is a major member of metabolic organization [34].The CD36-mediated uptake of FAs accounts for more than half of the uptake of all FAs in adipose and muscle tissue in mice [35].In humans CD36mediated fatty acid uptake is also important, and FA uptake is significantly reduced by CD36 deficiency [36].CD36 was identified in tumor cell membranes and is involved in the uptake of fatty acids by the surface membrane in a variety of malignant tumor cells; The knockdown of CD36 expression in mouse models completely ceases the aggressive metastatic behavior of tumor cells [37].CD36 is also expressed at higher levels in tumor-infiltrating regulatory T cells, and inhibition of CD36 blocks the adaptation of regulatory T cells to the tumor microenvironment and enhances antitumor activity [38].
In conclusion, the specific phenotype of aberrant fatty acid metabolism in tumor cells is gradually attracting great attention, and exploring the role of aberrant fatty acid metabolism in the development of breast cancer and strategies to treat malignant tumors by targeting fatty acid metabolic pathways is gaining attention.

Chemotherapy
The response and resistance of tumor cells to chemotherapeutic agents have been thought to be associated with alterations in the lipid composition of cell membranes.According to preclinical data, one of the characteristics of the resistance by tumor cells to chemotherapy drugs was the decrease in the fluidity of the membrane lipid bilayer.The decrease in fluidity is due to changes in the proportion of saturated fatty acyl chains in the lipid composition of the cell membrane, which plays a leading role, especially in lipid tumor cells.Due to the reduced fluidity, the drug is blocked from entering tumor cells through passive diffusion or endocytosis, and the absorption may be destroyed [39,40].Furthermore, it leads to enhanced formation of membrane domains in resistant regions that activate membrane-bound ATP-binding cassettes (ABCs) multidrug efflux transporters, thereby promoting a multidrug resistance phenotype that affects anticancer drugs other than chemotherapy [41,42].Interestingly, the regulation of membrane fluidity by supplementing polyunsaturated fatty acids can change the ABC-mediated drug efflux, which indicates that lipid modulators or dietary intervention may be an effective strategy for chemo-sensitization.
Since chemoresistant cancer cells have a relatively low ratio of polyunsaturated and saturated fatty acids in their cells, they are less sensitive to toxic lipid peroxidation in response to many chemotherapeutic drugs induced by oxidative stress, compared to sensitive cancer cells, thus, inhibit cell apoptosis and ferroptosis [40,43].Lipid peroxidation is a major feature of ferroptosis, and glutathione peroxidase 4 (GPX4), which is a selenium-containing cysteine enzyme, eliminates lipid peroxidation and prevents cell ferroptosis [44,45].A reduction in lipid peroxidation sensitivity appears to be associated with enhanced antioxidant defense, which is characteristic of chemoresistant cancer cells.
As more evidence suggests that membrane changes are associated with chemotherapy drug resistance, key pathways, and enzymes that drive changes in lipid distribution in tumor cells have become potential targets for improving chemotherapy sensitivity.Thus, fatty acid synthasesmajor members of fatty acid metabolism-can alter the susceptibility of many types of cancer cells to chemotherapy drugs, while ectopic overexpression of FASN in breast cancer cells can generate widespread chemotherapy resistance in vitro [46].The chemical sensitization of the FA inhibitor orlistat is related to a decrease in the expression of the multidrug resistance protein, thereby indicating that the change in membrane composition may be important.In a phase I clinical trial, oral FASN inhibitor TVB-2640 reverses taxane resistance in breast cancer with an acceptable toxicity and has potential clinical application [47].
Targeting fatty acid oxidation as a chemo-sensitization strategy has also received attention, as it promotes the survival of tumor cells by generating energy and maintaining redox homeostasis.There are significant differences in the expression of CPT1B and CPT1C in recurrent breast cancer and chemotherapy-resistant breast cancer tumor tissues or cell lines compared to primary cancer cells [15,48].Indeed, inhibition of fatty acid oxidation using CPT1 inhibitors resulted in sustained chemo-sensitization of tumor cells [15].
Lipid droplet accumulation is another less-studied feature of chemoresistant cancer cell lines [49][50][51].Lipid droplets can serve as an additional source of oxidation of fatty acids under conditions of metabolic stress or as a "sink" for isolating hydrophobic drugs [52], thereby directly contributing to chemoresistance.Likewise, in a model of chemoresistance, breast cancer cell lines were filled with smaller but functional mitochondria and numerous lipid droplets compared to parental cells [51].Subsequent analysis of these cell lines and clinical chemotherapyresistant breast cancer cells revealed an enhanced expression of the lipid droplet locus protein recombinant perilipin 4 (PLIN4), a perilipin coating on the observed lipid droplets, which is involved in the mobilization of lipid droplet fatty acids.The transcriptional silencing of PLIN4 reduces the activity of drug-resistant cells but has no effect on drug-sensitive cells, which indicates that targeting PLIN4 may be able to reverse breast cancer chemoresistance.

Targeted Therapy
The use of anti-HER2 targeted therapy can increase the survival of HER2+ breast cancer patients; however, some patients cannot get released from the therapy and some patients develop drug resistance.The intake of essential fatty acids can affect anti-HER2 efficacy and ALA and docosahexaenoic acid, as omega-3 fatty acids, modulate the HER2-associated lipogenic phenotype, induce cell apoptosis, and increase anti-HER2 therapy in HER2overexpressed breast cancer cells [53].From the combinational models of the oncoinformatic, pharmacodynamic, and metabolic parameters, long-chain omega-3 fatty acids are potential targeted therapeutics for HER2-positive breast cancer treatment [54].During the anti-HER2 treatment, modification of nutrient intake during treatment is also a potential strategy to improve the efficacy of drug therapy.
The finding that HER2 signaling pathways activate the expressions of FAs to promote cancer cell proliferation [55,56] and that there is bidirectional crosstalk between these pathways suggests that inhibiting FAs is a reasonable strategy to conquer the acquired resistance to HER2-targeted therapeutics in preclinical cancer models [57].An experiment on tumor-bearing mice showed that the FASN inhibitor G28UCM alone or in combination with trastuzumab can significantly improve the anticancer effect of HER2+ breast cancer or trastuzumab-resistant HER2+ breast cancer without the same side effects caused by βoxidation cross-activation [57].Survival of cancer cells by suppressing the PI3K pathway increases the size and number of lipid droplets and fatty acid oxidation, thereby maintaining cell survival and tumor proliferation [58].
In vitro, the most striking feature of breast cancer cell resistance to lapatinib (HER2 and EGFR inhibitor) is the upregulation of CD36, and in turn, the uptake of fatty acids is accompanied by lipid droplet accumulation.After clinical patients received HER2-targeted therapy, the expression of CD36 in clinical breast cancer tissues was also significantly increased, and the clinical prognosis of HER2positive breast cancer with higher CD36 levels was poor, indicating that fatty acid uptake and metabolism were related to anti-HER2 targeted therapy resistance [59].Resensitization of lapatinib-resistant cells and the induction of apoptosis by knockdown or pharmacological inhibition of CD36.The role of CD36 in lapatinib resistance was also supported by mouse tumor transplantation models, whereby anti-CD36 antibodies significantly sensitized drug-resistant tumors to lapatinib [60].

Endocrine Therapy
Hormones such as estrogens and androgens act in concert with their anabolic effects, profoundly affecting their target tissues as well as lipid metabolism in hormonedependent breast cancers.The positive efficacy of endocrine therapy in the treatment of locally recurrent or metastatic diseases reflects the dependence of hormone receptor-positive breast cancer cells on these hormonesignaling pathways, although endocrine resistance frequently occurs.Analysis of endocrine-resistant breast cancers has shown prominent features of lipid metabolism upregulation in transcriptomic or proteomic comparisons [61,62].Studies of lipid-modifying drugs with endocrine therapies have shown good preclinical efficacy, yet these observations lack clinical support.
In ER-positive breast cancer, the interaction between estrogen signaling and lipid metabolism is not clear because of the presence of two homologous receptors (ERα and ERβ), each with a different transcriptional program, and multiple molecular disease subtypes (for which there are no adequate metabolic models).Compared to isogenic sensitive lines, a common characteristic of endocrine-resistant breast cancer cell lines is that SREBP1 drives the upregulation of genes related to lipid synthesis, and targeting SREBP1 effectively inhibits the proliferation of these drugresistant cell lines [50,62], which may be a potential clinical mechanism for the acquired drug resistance.Another study found that fatty acid oxidation and autophagy pathways were activated in tamoxifen-acquired drug-resistant breast cancer cells.Although the PI3K/AKT/mTOR pathway is a potential target for endocrine-resistant patients and can reverse some endocrine-resistant patients in the clinic, there are complex signal feedback loops that can counteract the impact of targeting this pathway [63].In endocrine-resistant cells, the simultaneous inhibition of PI3K/AKT/mTOR and FAO/autophagy is potentially required for re-sensitizing the cells for endocrine therapy.The CPT1A-FAO signaling axis, which is important for fatty acid metabolism, is also essential for endocrineresistant breast cancer cells, maybe as a potential combinatory therapy against endocrine resistance in ER+ breast cancer [64].

Immunotherapy
Fatty acid metabolism has potential prognostic significance in cancer and has an impact on the remodeling of the tumor microenvironment and the efficacy of immunotherapy [65].Regulatory T lymphocytes are essential for human immune tolerance but can also promote immunosuppression and immune escape from the tumor microenvironment, thereby diminishing the efficacy of tumor immunotherapy.Moreover, it has been reported that lipid signaling promotes the functional specialization of tumorregulating T lymphocytes.The study shows that inhibition of lipid synthesis and metabolic signaling depends on the SREBP of regulatory T lymphocytes to generate an effective antitumor immune response without autoimmune toxicity [66].This study also found that SREBP activity was significantly higher in tumor-regulating T lymphocytes for human breast or head and neck squamous cell carcinomas.In addition, the deletion of the SREBP cleavage activator protein, which is required for SREBP activity in these cells, inhibited tumor growth and enhanced the immunotherapeutic effect of PD-(L)1 inhibitors targeting programmed death proteins on the surface of killer T lymphocytes.The de novo synthesis of fatty acids from fatty acid synthase contributes to the functional maturation of regulatory T lymphocytes [67], while the absence of fatty acid synthase from regulatory T lymphocytes can inhibit tumor growth.
FASN has recently been found to be associated with increased PD-L1 expression, thereby suggesting a role for fatty acids in the impairment of the immune response in the tumor microenvironment [68].Orlistat, an agent used in the treatment of obesity, has been reported to reduce FASN activity, as well as tumor growth activity in different can-cer models.The drug produced a significant decrease in PD-L1 expression.These findings suggest that orlistat interferes with different mechanisms involved in the control of tumor cell growth and may contribute to the reduction of tumor-associated immunopathogenesis.It was also found that the tumor microenvironment was altered in PD-(L)1acquired resistant tumor cell lines.Furthermore, Serpinf1, a protein that codes for pigment epithelial-derived factor (PEDF) was further explored.Serpinf1 overexpression was associated with increased production of free fatty acids and reduced activation of CD8+ cells.Conversely, the drug reduced free fatty acid production and reversed resistance to anti-PD-(L)1 therapy [69].
PD-1 signaling alters metabolic reprogramming and contributes to fatty acid utilization in β-oxidation [70], while changes in fatty acid metabolism have been identified in the mechanisms related to PD-(L)1 resistance.The reprogramming of lipid metabolism strengthens the functional differentiation of T cells in tumors and drugs that regulate lipid metabolism can reverse resistance to PD-(L)1 therapy, all of which provide new methods to target these cells for cancer treatment.

Drug Delivery System
Nanocarrier-based drug delivery systems have been widely used in the clinic to improve drug targeting and effective drug concentration.Lipid-based drug delivery has also found excellent application in the treatment of breast cancer.Drugs already in clinical use include liposomal adriamycin and liposomal paclitaxel, which can improve therapeutic efficacy and reduce toxic side effects.By changing the fatty acid composition in nanostructured lipid carriers, structural modifications, such as docosahexaenoic acid-based nanostructured lipid carriers [71], linoleic acidcoupled chitosan nanoparticles [72], and palmitate albumin nanoparticles [73] can influence drug delivery, alter drug sensitivity, reverse therapeutic resistance, and reduce toxic side effects.The combination of nanostructured lipid carriers with raloxifene, a previous endocrine therapeutic drug due to poor oral bioavailability, has enhanced the bioavailability and improved antitumor efficacy.

Conclusion
Owing to the specificity of the tumor microenvironment of adipocyte infiltration around breast cancer, a growing number of studies recognize the impact of fatty acid metabolism on tumor progression.Fatty acid homeostasis can alter cell viability and change lipid membrane composition, thereby affecting membrane fluidity and permeability, leading to tumor cell motility and metastasis.Fatty acid metabolism has also been associated with acquired resistance to a variety of anti-breast cancer drugs.Importantly, numerous studies have recently pointed out that combination therapeutic strategies targeting fatty acid metabolism to overcome therapeutic resistance may be a viable approach for the future, especially in the context of abnormal lipid metabolism and metabolic dysfunction.Finally, we believe that the use of tumor genomic testing in combination with classifying fatty acids in tumor cells, the microenvironment, and daily diets, to design more comprehensive precision medicine strategies, will be the direction to improve the prognoses of breast cancer patients.

Fig. 1 .
Fig. 1.The classification of fatty acids and their metabolism.Non-essential fatty acids can be synthesized in the body, although essential fatty acids can only be synthesized in plants.The figure also shows the relationships between the major fatty acids and the enzymes involved in their metabolism.Acetyl coenzyme A, which acts as a bridge among the three major energy substances (carbohydrates/proteins/fatty acids), is involved in the de novo synthesis pathway of fatty acids, while beta-oxidation of fatty acids also produces acetyl coenzyme A.