IMR Press / FBL / Volume 27 / Issue 9 / DOI: 10.31083/j.fbl2709258
Open Access Original Research
Forensic Characteristics and Genetic Background Dissections of Chinese Manchu and Zhuang Groups Using 59 Autosomal Diallelic InDels and 2 miniSTRs
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1 Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, 510515 Guangzhou, Guangdong, China
2 Microbiome Medicine Center, Department of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, 510280 Guangzhou, Guangdong, China
3 School of Forensic Medicine, Kunming Medical University, 650500 Kunming, Yunnan, China
4 Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, 710000 Xi’an, Shaanxi, China
*Correspondence: zhubofeng@i.smu.edu.cn (Bofeng Zhu)
These authors contributed equally.
Academic Editor: Said El Shamieh
Front. Biosci. (Landmark Ed) 2022, 27(9), 258; https://doi.org/10.31083/j.fbl2709258
Submitted: 6 June 2022 | Revised: 24 June 2022 | Accepted: 22 July 2022 | Published: 6 September 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Background: The latest development in molecular biology has offered an opportunity to construct multiplex panel with better applicability for forensic purpose, and a self-developed 64-plex panel, including 59 autosomal diallelic InDels, 2 miniSTRs, 2 Y-InDels, and an Amelogenin gene, was validated to be an effective forensic tool in the previous study. Methods: By applying the 64-plex panel for DNA profiling, the obtained genotypes and the corresponding frequency data were used to investigate the forensic characteristics and population genetic structures of the Chinese Manchu group from the Inner Mongolia Autonomous Region and the Chinese Zhuang group from the Yunnan province. Results: The 64-plex panel was qualified to perform human identification and paternity testing with the combined powers of discrimination of 0.99999999999999999999999999758 and 0.99999999999999999999999999691; and cumulative probabilities of exclusion of 0.99999866 and 0.99999880 in the studied Manchu and Zhuang groups, respectively. Relatively closer genetic relationships were found between the Chinese Manchu group and Han population in Beijing; and between the Chinese Zhuang group and Vietnamese Kinh population. Conclusions: It could be indicated from the results that, with the preliminary ability to distinguish ancestral components from all the studied groups, the 64-plex panel can not only serve as a robust forensic panel in the Manchu and Zhuang groups, but also offer genetic insights into the genetic differentiations and substructures of these populations.

Keywords
insertion/deletion polymorphism
miniSTR
Manchu
Zhuang
forensic efficiency
population genetics
Figures
Fig. 1.
Funding
81930055/ National Natural Science Foundation of China
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