IMR Press / FBL / Volume 27 / Issue 6 / DOI: 10.31083/j.fbl2706186
Open Access Original Research
Genome-Wide Analysis and Expression Profiling of SlHsp70 Gene Family in Solanum lycopersicum Revealed Higher Expression of SlHsp70-11 in Roots under Cd2+ Stress
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1 School of Agriculture, Forestry and Food Engineering, Yibin University, 644000 Yibin, Sichuan, China
2 Department of Plant Pathology, College of Agriculture, Guizhou University, 550025 Guiyang, Guizhou, China
3 Faculty of Pharmacy, Ahram Canadian University, 12451 Giza, Egypt
4 Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, University of Tennessee Institute of Agriculture, Knoxville, TN 37996, USA
5 Joint Institute for Biological Science, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA
6 College of Horticulture, Northwest A&F University, 712100 Yangling, Shaanxi, China
7 Genetics Department, Faculty of Agriculture, Zagazig University, 44519 Zagazig, Egypt
8 College of Agronomy, Northwest A&F University, 712100 Yangling, Shaanxi, China
9 Institute of Molecular Biology and Biotechnology, The University of Lahore, 54590 Lahore, Pakistan
10 Department of Botany, Government College University, 54000 Faisalabad, Pakistan
11 Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, 100107 Beijing, China
12 State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, 100091 Beijing, China
13 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081 Beijing, China
14 College of Biological Sciences and Biotechnology, Beijing Forestry University, 100107 Beijing, China
*Correspondence: ahmed_elsappah2006@yahoo.com (Ahmed H. El-Sappah); liyunzhou2007@126.com (Yunzhou Li); jia.li16@aliyun.com (Jia Li)
These authors contributed equally.
Academic Editor: Changsoo Kim
Front. Biosci. (Landmark Ed) 2022, 27(6), 186; https://doi.org/10.31083/j.fbl2706186
Submitted: 16 December 2021 | Revised: 1 April 2022 | Accepted: 15 April 2022 | Published: 9 June 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Background: Tomato is an important part of daily food, rich source of multitude nutrients, suitable candidate for bio-pharmaceutical production due to berry size and has numerous health benefits. Transcriptional regulation of metalloregulatory heat shock protein-70 family plays pivotal role in plants tolerance against abiotic stress factors including salinity, heat, cold, drought and trace metal elements such as cadmium (Cd2+). Methods: Here, we provide comprehensive report on in-silico identification of SlHsp70 family genes in tomato (Solanum lycopersicum) and their expression in tomato via qPCR analysis under broad range of trace metal elements. Results: In-silico analysis revealed 23 SlHsp70 family genes in tomato, phylogenetically divided into four groups I–IV and displayed expression in all tissues. Gene Ontology (GO) analysis revealed that SlHSP70 proteins were membrane localized which were involved in metal ions translocation and oxidoreductase activity to counter hyper-accumlation of reactive oxygen species (ROS). Conclusions: Cd2+ is a widespread heavy metal soil contaminent which is continously polluting fertile soils, a knotty issue which has serious implications over photosynthesis, nitrogen assimilation, minerals and water absorption by plants. Plants exposure to Cd2+ and subsequent qRT-PCR analysis revealed increased expression of SlHsp70-11 in tomato roots, which can be employed in breeding low Cd2+ enriched tomato varieties.

Keywords
SlHsp70
Solanum lycopersicum
RNA-seq
qRT-PCR
trace metal elements stresses
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