†These authors contributed equally.
Academic Editor: Taeg Kyu Kwon
Background: Endoplasmic reticulum stress (ERS) is a key part
of the apoptotic cascade that is initiated after cerebral ischemia-reperfusion
injury and is very important for research on poststroke rehabilitation. In
addition, the unfolded protein response (UPR) plays an important role in ERS
because it activates downstream apoptotic signal transduction and induces
apoptosis through the glucose-regulated protein 78 (GRP78)/protein kinase R
(PKR)-like ER kinase (PERK)/activating transcription factor 4 (ATF4) pathway. The
Gua Lou Gui Zhi Decoction (GLGZD) ameliorated neuronal apoptosis of
ischemia-reperfusion injury caused by middle cerebral artery occlusion (MCAO) had
been proved in our previous study. The present study aims to underly the
regulatory ability of GLGZD in ERS-induced apoptosis mediated by the
GRP78/PERK/ATF4 pathway. Methods: GLGZD was analyzed by HPLC. The
effects of GLGZD were obversed on MCAO-induced ischemic rats. The cerebral
infarct volume was detected by 2,3,5-Triphenyl-2H-Tetrazolium Chloride (TTC) Staining. Terminal Deoxynucleotidyl Transferase-Mediated dUTP-Biotin Nick End Labeling (TUNEL) were used to detect apoptosis.
Transmission Electron Microscopy (TEM), Ca