IMR Press / FBL / Volume 23 / Issue 12 / DOI: 10.2741/4702

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

The function of replication and SCF complex during Drosophila wing development

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1 Faculty of Medicine, Mie University, Tsu, Mie, 514-8507, Japan
Front. Biosci. (Landmark Ed) 2018, 23(12), 2235–2244; https://doi.org/10.2741/4702
Published: 1 June 2018
Abstract

Chromosomal DNA replication machinery functions in the growing cells and organs in multicellular organisms. We previously demonstrated that its knockdown in several tissues of Drosophila led to a rough eye phenotype, the loss of bristles in the eye and female sterile. In this paper, I investigated in detail the wing phenotype using RNAi flies, and observed that the knockdown not only of Mcm10 but also of some other prereplicative complex components including Cdt1, Polα-primase, RPA, Psf2 (partner of SLD five 2; a subunit of GINS (Go, Ichi, Nii, and San; five, one, two, and three in Japanese) and Rfc3 (replication factor C 3; a subunit of RFC complex) demonstrated wing phenotypes, using Gal4-driver flies. Surprisingly, some SCF complex components, which control cell cycle progression via protein degradation, also showed the wing phenotype. These results showed that the DNA replication machinery contributes to wing development independently of growth, probably through defects in DNA replication and protein degradation at specific places and times.

Keywords
DNA replication
Prereplicative Complex
SCF complex
Drosophila
development
E2F-DP1
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