IMR Press / FBL / Volume 22 / Issue 6 / DOI: 10.2741/4529

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Review

Current strategies towards therapeutic manipulation of mtDNA heteroplasmy

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1 Department of Neurology, University of Miami Miller School of Medicine, Miami, FL 33136, USA

Academic Editor: Mikhail F Alexeyev

Front. Biosci. (Landmark Ed) 2017, 22(6), 991–1010; https://doi.org/10.2741/4529
Published: 1 January 2017
(This article belongs to the Special Issue Mitochondrial DNA)
Abstract

Mitochondrial disease is a multifactorial disorder involving both nuclear and mitochondrial genomes. Over the past 20 years, great progress was achieved in the field of gene editing which raised the possibility of partial or complete elimination of mutant mtDNA that causes disease phenotypes. Each cell contains thousands of copies of mtDNA which can be either wild-type (WT) or mutant, a condition called heteroplasmy. As there are multiple copies of mtDNA inside a cell, the percentage of mutant mtDNA can vary and a directional shift in the heteroplasmy ratio towards an increase of WT mtDNA copies would have therapeutic value. Gene editing tools have been adapted to translocate to mitochondria and were able to change heteroplasmy in a predictable manner. These include mitochondrial targeted restriction endonucleases, Zinc-finger nucleases, and TAL-effector nucleases. These procedures could also be adapted to reduce the levels of mutant mtDNA in embryos, offering an option to the controversial mitochondrial replacement techniques during in vitro fertilization. The current strategies to induce heteroplasmy shift of mtDNA and its implications will be comprehensively discussed.

Keywords
Mitochondrial DNA
Heteroplasmy
Restriction Endonucleases
Mitotalens
mitoZFNs
Review
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