IMR Press / FBL / Volume 22 / Issue 11 / DOI: 10.2741/4574

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.


Remodeling of tick cytoskeleton in response to infection with Anaplasma phagocytophilum

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1 Institute of Parasitology, Biology Center, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic
2 Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic
3 SaBio Instituto de Investigacion en Recursos Cinegeticos IREC (CSIC-UCLM-JCCM), 13005 Ciudad Real, Spain
4 Department of Virology, Veterinary Research Institute, Hudcova 70, CZ-62100 Brno, Czech Republic
5 Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 USA
Front. Biosci. (Landmark Ed) 2017, 22(11), 1830–1844;
Published: 1 March 2017
(This article belongs to the Special Issue From tick genetics to genomics)

The obligate intracellular pathogen Anaplasma phagocytophilum infects vertebrate and tick hosts. In this study, a genome-wide search for cytoskeleton components was performed in the tick vector, Ixodes scapularis. The available transcriptomics and proteomics data was then used to characterize the mRNA and protein levels of I. scapularis cytoskeleton components in response to A. phagocytophilum infection. The results showed that cytoskeleton components described in other model organisms were present in the I. scapularis genome. One type of intermediate filaments (lamin), a family of septins that was recently implicated in the cellular response to intracellular pathogens, and several members of motor proteins (kinesins and dyneins) that could be implicated in the cytoplasmic movements of A. phagocytophilum were found. The results showed that levels of tubulin, actin, septin, actin-related proteins and motor proteins were affected by A. phagocytophilum, probably to facilitate infection in I. scapularis. Functional studies demonstrated a role for selected cytoskeleton components in pathogen infection. These results provided a more comprehensive view of the cytoskeletal components involved in the response to A. phagocytophilum infection in ticks.

Ixodes scapularis
Anaplasma phagocytophilum
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