IMR Press / FBL / Volume 21 / Issue 4 / DOI: 10.2741/4417

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Higher cardiogenic potential of IPSCs derived from cardiac versus skin stromal cells

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1 Laboratory of Vascular Biology and Regenerative Medicine Centro Cardiologico Monzino IRCCS, Milano, 20138 Italy
2 Center for Biomedicine, European Academy Bozen/Bolzano (EURAC) (affiliated institute of the University of Lübeck), Bolzano, 39100 Italy
3 Del E. Webb Center for Neuroscience, Aging & Stem Cell Research, Sanford-Burnham-Prebys Medical Discovery Institute, La Jolla, California 92037, USA
4 Laboratory of Immunology and Functional Genomics, Centro Cardiologico Monzino IRCCS, Milano, 20138 Italy
5 The PaceLab, Department of Biosciences, University of Milano, Milano, 20133 Italy
6 Department of Pharmacological and Biomolecular Sciences, University of Milano, Milano, 20133 Italy
7 Department of Electrical, Computer and Biomedical Engineering, University of Pavia, Pavia, 27100 Italy
8 Laboratorio Specialistico di Ematologia, Ospedale Centrale di Bolzano, Azienda Sanitaria dell’Alto Adige, Bolzano, 39100 Italy
9 CERT, Center of Excellence for Toxicological Research, INAIL, ex ISPESL, University of Parma, Parma, 43125 Italy
10 Humanitas Clinical and Research Center, Rozzano (Milano), 20089 Italy
11 Division of Cardiovascular Epigenetics, Department of Cardiology, Goethe University, Frankfurt am Main, 60590 Germany
12 Department of Clinical Sciences and Community Health, University of Milano, Milano, 20122 Italy
13 Centro Interuniversitario di Medicina Molecolare e Biofisica Applicata (CIMMBA), University of Milano, Milano, 20133 Italy
14 Departments of Medicine/Cardiology, University of California-San Diego (UCSD), La Jolla, California 92037, USA
Academic Editor:Elena De Falco
Front. Biosci. (Landmark Ed) 2016, 21(4), 719–743; https://doi.org/10.2741/4417
Published: 1 January 2016
(This article belongs to the Special Issue Stem cells: in health and diseases)
Abstract

Prior studies have demonstrated that founder cell type could influence induced pluripotent stem cells (iPSCs) molecular and developmental properties at early passages after establishing their pluripotent state. Herein, we evaluated the persistence of a functional memory related to the tissue of origin in iPSCs from syngeneic cardiac (CStC) vs skin stromal cells (SStCs). We found that, at passages greater than 15, iPSCs from cardiac stromal cells (C-iPSCs) produced a higher number of beating embryoid bodies than iPSCs from skin stromal cells (S-iPSCs). Flow cytometry analysis revealed that dissected beating areas from C-iPSCs exhibited more Troponin-T positive cells compared to S-iPSCs. Beating areas derived from C-iPSCs displayed higher expression of cardiac markers, more hyperpolarized diastolic potentials, larger action potential amplitude and higher contractility than beaters from skin. Also, different microRNA subsets were differentially modulated in CStCs vs SStCs during the reprogramming process, potentially accounting for the higher cardiogenic potentials of C-iPSCs vs S-iPSCs. Therefore, the present work supports the existence of a founder organ memory in iPSCs obtained from the stromal component of the origin tissue.

Keywords
Induced Pluripotent Stem Cells
Stromal Cells
Fibroblasts
Cardiomyogenic Differentiation
Cardiomyocytes
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