IMR Press / FBL / Volume 21 / Issue 2 / DOI: 10.2741/4392

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Differential proteome analysis along jejunal crypt-villus axis in piglets

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1 Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center of Healthy Breeding Livestock and Poultry, Human Engineering and Research Center of Animal and Poultry Science, Key Lab Agroecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha City, Hunan, 410125, People’s Republic of China, P. R. China
2 School of Life Sciences, Hunan Normal University, Changsha 410008, P. R. China
3 State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, People’s Republic of China 100193, P. R. China
4 Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, People’s Republic of China, P. R. China
5 Department of Animal Science, Texas A and M University, College Station, TX 77843, USA
6 Hunan agricultural university, Changsha, Hunan P.R. China
7 Fujian Aonong Biotechnology Corporation, Xiamen, Fujian, 361007, P. R. China
Academic Editor:Guoyao Wu
Front. Biosci. (Landmark Ed) 2016, 21(2), 343–363; https://doi.org/10.2741/4392
Published: 1 January 2016
(This article belongs to the Special Issue Amino acids in nutrition, health, and disease)
Abstract

The brush-border membrane (BBM) of enterocytes is responsible for the digestion and absorption of nutrients and ions in the small intestine. To identify the BBM proteins involved in epithelial cell maturation along the crypt-villus axis, enterocytes were sequentially isolated from the villus tip to the crypt of the jejunum from 21-day-old suckling piglets. After preparation of BBM vesicles, we detected 194 proteins in the jejunal epithelial cells by isobaric tags using relative and absolute quantification (iTRAQ) techniques. Of these, 56 BBM proteins were differentially expressed along the crypt-villus axis. During differentiation, the expression of proteins related to digestion and absorption of nutrients was primarily downregulated at the upper, middle villus, or crypt compared to the villus tip, while expression of proteins related to structural and enzyme regulator proteins was largely upregulated. We verified the differences in Na+/K+ -transporting ATPase, galectin-3, and an intestinal-type fatty acid binding protein by western blot or immunochemical analysis. Identification of BBM-associated proteins helps enhance our understanding of digestion and absorption in piglets and other mammals, including humans.

Keywords
Small Intestine
Proteomics
Villus-Crypt Axis
Suckling
Piglet
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