IMR Press / FBL / Volume 13 / Issue 9 / DOI: 10.2741/2943

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Vector-based delivery of siRNAs: in vitro and in vivo challenges

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1 Institute for Cancer Research, Department of Immunology, Molecular Medicine Group, Rikshospitalet-Radiumhospitalet Medical Centre, Montebello 0310 Oslo, Norway

*Author to whom correspondence should be addressed.

Academic Editor: Mouldy Sioud

Front. Biosci. (Landmark Ed) 2008, 13(9), 3488–3493;
Published: 1 May 2008
(This article belongs to the Special Issue RNA interference: from basic to medical applications)

RNA interference (RNAi) induced by small interfering RNAs (siRNAs) has recently become a powerful tool to knock-down gene expression in a sequence-specific manner. In addition to chemically made siRNAs, stable expression of siRNA in the form of short hairpin RNAs (shRNAs) expressed from an RNA polymerase III (pol III) promoter is now widely used approach for the application of RNAi in mammalian cells. However, long-term suppression using constitutive promoters can be problematic and emerging evidence indicates that siRNAs can cause several side effects in human cells. Here we review the recent advances in developing controllable expression vectors in order to accelerate the therapeutic applications of RNAi.

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