IMR Press / FBL / Volume 13 / Issue 18 / DOI: 10.2741/3210

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Article
Notch is activated in RANKL-induced osteoclast differentiation and resorption
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1 Department of Orthodontics, University Medical Centre Groningen, University of Groningen, The Netherlands
2 Groningen University Institute for Drug Exploration, University Medical Centre Groningen, University of Groningen, The Netherlands
3 Department of Pathology and Laboratory Science, Universiy Medical Centre Groningen, University of Groningen, The Netherlands
4 Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, China

*Author to whom correspondence should be addressed.

Front. Biosci. (Landmark Ed) 2008, 13(18), 7064–7071; https://doi.org/10.2741/3210
Published: 1 May 2008
Abstract

The process of osteoclast differentiation and resorption is fine-tuned by signal pathways, which need to be further elucidated. The aim of this study was to explore the possible connections between NF-κB and Notch in RANKL-induced osteoclast activity. To this end, RANKL was used to stimulate mouse osteoclast precursor cell line RAW264.7. The number of multinucleated TRAP+ osteoclasts was counted and the resorption area was measured. NF-κB transcriptional factor activity was determined by EMSA. Quantitative RT-PCR and Western blotting analysis were used to determine Hes1 (one of Notch signaling primary targets) mRNA and protein expressions respectively. Mature osteoclasts and bone resorption areas were detected in the present study. NF-κB activity was increased in RANKL-induced osteoclast differentiation and resorption. mRNA and protein expressions of Hes1 in RAW264.7 cells were up-regulated after RANKL stimulation. In conclusion, NF-κB signaling mediated RANKL-induced osteoclast differentiation and resorption, during which, Notch signaling was activated. Therefore, Notch could be a novel therapeutic target for bone resorption related diseases.

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