IMR Press / FBL / Volume 13 / Issue 15 / DOI: 10.2741/3120

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Unreplicated DNA in mitosis precludes condensin binding and chromosome condensation in S. cerevisiae

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1 Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA
2 University of Plovdiv, Plovdiv, Bulgaria
3 Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, London, UK

*Author to whom correspondence should be addressed.

Academic Editor: Kenichi Yoshida

Front. Biosci. (Landmark Ed) 2008, 13(15), 5838–5846;
Published: 1 May 2008
(This article belongs to the Special Issue Cell division cycle-associated genes in a chromatin context)

Condensin is the core activity responsible for chromosome condensation in mitosis. In the yeast S. cerevisiae, condensin binding is enriched at the regions where DNA replication terminates. Therefore, we investigated whether DNA replication completion determines the condensin-binding proficiency of chromatin. In order to fulfill putative mitotic requirements for condensin activity we analyzed chromosome condensation and condensin binding to unreplicated chromosomes in mitosis. For this purpose we used pGAL:CDC6 cdc15-ts cells that are known to enter mitosis without DNA replication if CDC6 transcription is repressed prior to S-phase. Both the condensation of nucleolar chromatin and proper condensin targeting to rDNA sites failed when unreplicated chromosomes were driven in mitosis. We propose that the DNA replication results in structural and/or biochemical changes to replicated chromatin, which are required for two-phase condensin binding and proper chromosome condensation.

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