IMR Press / FBL / Volume 13 / Issue 1 / DOI: 10.2741/2684

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Initial signaling response to acute exercise bout is similar in hearts of rats bred for divergent exercise capacities
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1 Division of Kinesiology, Laboratory of Molecular Kinesiology, The University of Michigan, 401 Washtenaw Avenue, Ann Arbor, MI 48109-2214
2 Department of Physical Medicine and Rehabilitation, University of Michigan Medical School, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109-0718

Academic Editor: Ulrik Wisloff

Front. Biosci. (Landmark Ed) 2008, 13(1), 347–355; https://doi.org/10.2741/2684
Published: 1 January 2008
(This article belongs to the Special Issue Exercise in medicine: from basic science to clinical practice)
Abstract

Rats artificially selected as low capacity runners (LCR) exhibit features of the metabolic syndrome, and blunted exercise training-induced cardiac hypertrophy compared with high capacity runners (HCR). We tested the hypothesis that the divergent cardiac phenotypes may be due to diminished activation of signaling proteins in LCR vs HCR rats. LCR (n=18) and HCR (n=18) rats were randomly assigned to acute exercise or control groups. Ten minutes after a 10-min bout of high intensity treadmill exercise, rats were euthanized, and left ventricles (LV) were harvested. LV homogenates were immunoblotted for phosphorylated and total levels of extracellular regulated kinase (ERK1/2), c-Jun N-terminal kinase (JNK), p38, Akt, S6, and the ribosomal S6 protein kinases S6K and p90RSK. Alterations in protein ubiquitination were examined as an index of protein turnover. In LCR and HCR rats, S6 was activated to a similar extent after exercise (5-fold vs control), as were JNK1/2, p38, and ERK1/2 (each 1.5-fold). Exercise significantly reduced ubiquitination of some proteins, suggesting diminished post-exercise protein degradation. That no significant LCR/HCR differences were observed 10-min post-exercise in the signaling pathways studied herein suggests that the source of the differing cardiac phenotypes in LCR/HCR rats may involve differing activation times and/or other signaling pathways.

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