IMR Press / FBL / Volume 12 / Issue 5 / DOI: 10.2741/2178

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Identification of ICIS-1, a new protein involved in cilia stability
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1 Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Universite Paris 7, France
2 Sanofi-Aventis, Labege, France
3 UMR 8080, Centre National de la Recherche Scientifique/Universite Paris Sud, Orsay, France
4 Ecole Superieure de Physique et Chimie Industrielles, Paris, France
Front. Biosci. (Landmark Ed) 2007, 12(5), 1661–1669;
Published: 1 January 2007

Cilia are specialized organelles that exert critical functions in numerous organisms, including that of cell motility, fluid transport and protozoan locomotion. Ciliary architecture and function strictly depend on basal body formation, migration and axoneme elongation. Numerous ultrastructural studies have been undertaken in different species to elucidate the process of ciliogenesis. Recent analyses have led to identification of genes specifically expressed in ciliated organisms, but most proteins involved in ciliogenesis remain uncharacterized. Using human nasal epithelial cells capable of ciliary differentiation in vitro, differential display was carried out to identify new proteins associated with ciliogenesis. We isolated a new gene, ICIS-1 (Involved in CIlia Stability-1), upregulated during mucociliary differentiation. This gene is localized within the TGF-beta1 promoter and is ubiquitously expressed in human tissues. Functional analyses of gene expression inhibition by RNA interference in Paramecium tetraurelia indicated that the ICIS-1 homologue interfered with cilia stability or formation. These findings demonstrate that ICIS-1 is a new protein associated with ciliated cells and potentially related to cilia stability.

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