IMR Press / FBL / Volume 12 / Issue 13 / DOI: 10.2741/2442

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Mobile group II intron targeting: applications in prokaryotes and perspectives in eukaryotes
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1 Sigma-Aldrich Biotechnology, Research and Development, 2909 Laclede Avenue, St. Louis, MO 63103, USA
Academic Editors:Eric Kmiec, Hetal Parekh-Olmedo
Front. Biosci. (Landmark Ed) 2007, 12(13), 4972–4985; https://doi.org/10.2741/2442
Published: 1 September 2007
(This article belongs to the Special Issue Targeted gene repair)
Abstract

Mobile group II introns are ribozymes and use a novel mechanism--target DNA-primed reverse transcription--to proliferate in DNA. Group II introns are a unique mobile element for their high sequence-specific, yet readily flexible target site recognition. Both the intron RNA and the intron-encoded protein (IEP) are involved in target site recognition, and the specificity is determined primarily by base pairing between the intron RNA and DNA target. Therefore, the intron RNA can be modified according to the desired target sequence for specific gene disruption. Group II intron knockout technology is mature in bacteria and is currently being developed in eukaryotes. This technology has great potential to revolutionize fields such as functional genomics, gene therapy, and cell line engineering.

Keywords
functional Genomics
Gene Therapy
Cell Line Engineering
Gene Knockout
Ribozyme
Gene Targeting
Homologous Recombination
Splicing
Reverse Splicing
Targetron
Reverse Transcriptase
Reverse Transcription
Double-Strand Break
Transposons
Review
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