IMR Press / FBL / Volume 11 / Issue 3 / DOI: 10.2741/1948

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Gene therapy of focal cerebral ischemia using defective recombinant adeno-associated virus vectors
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1 Department of Neurosurgery, Tri-Service General Hospital, Taipei, Taiwan
2 Department of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan
3 Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261
4 Department of Ophthalmology, Mackay Memorial Hospital, Taipei, Taiwan
Academic Editor:Cesario Borlongan
Front. Biosci. (Landmark Ed) 2006, 11(3), 2061–2070; https://doi.org/10.2741/1948
Published: 1 September 2006
(This article belongs to the Special Issue Gene therapy for stroke)
Abstract

This review presents our experience and results concerning cerebral stroke gene therapy with a rat model subjected to rAAV-vector delivered IL-1ra and GDNF. The methodology involving the production of high-titer recombinant adeno-associated virus vectors in the absence of helper adenovirus and the creation of a tri-vessel ligation model of focal ischemic cerebral stroke in rats are described in detail. Furthermore, a literature review of other viral vectors, murine models of focal cerebral ischemia and candidates for therapeutic transgenes used for cerebral stroke gene therapy are presented. Lastly, the potentials and limitations of stroke gene therapy are discussed adding an analysis of possibilities of future experiment designs.

Keywords
Brain
Nervous system
Neruron
Adenovirus
Adeno-associated virus
Cytokine
GDNF
IL-1Ra
Stroke
rat
Apoptosis
Review
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