IMR Press / FBL / Volume 10 / Issue 3 / DOI: 10.2741/1756

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

DNA hypomethylation of individual sequences in aborted cloned bovine fetuses
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1 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Science, Beijing 100080, China
2 Graduate School, Chinese Academy of Sciences, Beijing 100080, China
3 Department of Veterinary Pathobiology, University of Missouri-Columbia, Columbia, Missouri 65211
Front. Biosci. (Landmark Ed) 2005, 10(3), 3002–3008;
Published: 1 September 2005

Cloned bovines have a much higher abortion rate than those derived in vivo. Available evidence indicates that inappropriate epigenetic reprogramming of donor nuclei is the primary cause of cloning failure. To gain a better understanding of the DNA methylation changes associated with the high abortion rate of cloned bovines, we examined the DNA methylation status of a repeated sequence (satellite I) and the promoter regions of two single-copy genes (interleukin 3/cytokeratin) in aborted cloned fetuses, aborted fetuses derived from artificial insemination (AI), cloned adults and AI adults by bisulfite sequencing and restriction enzyme analysis. Two of four aborted cloned fetuses show very low methylation levels in the two single-copy gene promoter regions. One of the two fetuses also showed undermethylated status in the satellite I sequence. The other two aborted cloned fetuses have similar methylation levels to those of aborted AI fetuses. However, no difference in methylation was observed between cloned adults and AI adults. Our results demonstrate for the first time the undermethylated status of individual sequences in aborted cloned fetuses. These findings suggest that aberrant DNA methylation may contribute to the developmental failure of cloned bovine fetuses.

DNA Methylation
Nuclear Transfer
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