IMR Press / FBL / Volume 1 / Issue 1 / DOI: 10.2741/A104

Frontiers in Bioscience-Landmark (FBL) is published by IMR Press from Volume 26 Issue 5 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Differential susceptibility to anti-receptor induced apoptosis in adult murine B-cells: role of B1 cells
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1 Immunology Department, American Red Cross Holland Laboratory, Rockville, MD 20855, USA
Academic Editor:Kenth Gustafsson
Front. Biosci. (Landmark Ed) 1996, 1(1), 39–45;
Published: 1 July 1996

We and others have recently found that mature murine B cells can be induced to undergo apoptosis, in vitro, in a dose-dependent manner, by extensive crosslinking of membrane IgM with polyclonal anti-mu. During the analysis of tolerance in transgenic mice expressing rearranged IgM or IgM+ IgD receptors, we observed that, Sp6 anti-TNP Ig and anti-MHC transgenic splenocytes, would undergo receptor-mediated apoptosis in vitro just like their normal, non-transgenic littermates. However, transgenic mice expressing rearranged receptors typical of B1 cells, not only contained large numbers of CD5+ cells in their spleens, but these cells failed to undergo apoptosis under conditions that led to programmed cell death in normal splenocytes. B1 spleen cells also failed to proliferate with anti-IgM, although the responsiveness of cells from the other transgenic lines varied depending on the background strains. These differences are due in part, to strain differences, but they also imply that the response pattern of transgenic B cells reflects not only the subset composition in this organ, but also the transgenic specificity of the receptor.

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