IMR Press / FBE / Volume 6 / Issue 1 / DOI: 10.2741/E693

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
microRNAs are dysregulated in the cerebral microvasculature of CKD mice
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1 INSERM U1088, Rue des Louvels, F-80037, Amiens, France, Faculty of Pharmacy and Medicine, University of Picardie Jules Verne, Rue des Louvels, F-80037, Amiens, France
2 University Paris 13, UFR SMBH, 74 rue Marcel Cachin, F-93017 Bobigny, France
3 Division of Pharmacology, Amiens University Hospital, F-80054, Amiens, France
4 Division of Nephrology, Ambroise Pare Hospital, Paris Ile de France Ouest (UVSQ) University, 09 avenue Charles de Gaulle 92100 Boulogne Billancourt cedex, France
5 Centre De Biologie Humaine (CBH), Amiens University Hospital, F-80054, Amiens, France

*Author to whom correspondence should be addressed.

Academic Editor: Allal Ouhtit

Front. Biosci. (Elite Ed) 2014, 6(1), 80–88; https://doi.org/10.2741/E693
Published: 1 January 2014
(This article belongs to the Special Issue Oxidative stress and cardiovascular diseases)
Abstract

Vascular calcification arises during chronic kidney disease (CKD), and increases the risk of cardiovascular mortality. In CKD, alterations of cerebral circulation were linked with an increase in ischemic strokes and behavioral troubles. Studying pathophysiological mechanisms of calcifications and detecting new biomarkers in the cerebral circulation is thus an important issue. microRNAs are small non-coding, single-stranded RNAs that regulate messenger RNAs at the post-transcriptional level. They are involved in numerous pathologies and represent new opportunities to develop disease predictors. We used RT-qPCR to quantify endothelial-specific microRNAs in cerebral arterioles from WT mice and from pathological models of CKD. We used four mice groups: WT SHAM, WT CKD, Apolipoprotein E Knock-Out (ApoE-KO) SHAM, ApoE-KO CKD. Brains were removed after two and ten weeks of uremia and RNA from cerebral arterioles was extracted. miR-17 and miR-126 were the most dysregulated in the pathological conditions, at both the second week and tenth week of uremia. Our results suggest that miR-17 and miR-126 are potential new biomarkers of cerebral troubles of CKD patients and new therapeutic targets for innovative treatments.

Keywords
Cerebral arterioles
microRNA
Chronic kidney disease
Stroke
Endothelial cells
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