IMR Press / FBE / Volume 4 / Issue 1 / DOI: 10.2741/e366

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Effect of chemopreventive agents on differentiation of mouse embryonic stem cells

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1 Center of Physiology and Pathophysiology, Institute of Neurophysiology, Robert-Koch Str. 39, 50931 Cologne, Germany

*Author to whom correspondence should be addressed.

Academic Editor: Hala Gali-Muhtasib

Front. Biosci. (Elite Ed) 2012, 4(1), 156–168; https://doi.org/10.2741/e366
Published: 1 January 2012
(This article belongs to the Special Issue New emerging anticancer lead compounds from plants)
Abstract

Chemopreventive agents are derived from edible plants and from ancient time is a part of daily intake for many humans and animals. There are several lines of compelling evidence from epidemiological, clinical and laboratory studies that these dietary constituents are associated in reducing cancer risks. However, developmental toxicity of these natural compounds cannot be excluded. In the present study, we examined the effect of chemopreventive agents on the differentiation of mouse embryonic stem cells (ESCs) as an in vitro embryotoxicity model. We assumed that inhibition of developmentally regulated genes in vitro might predict developmental toxicity also under in vivo conditions. We found that epigallocatechin gallate (EGCG) (20 µM) induced the expression of mesodermal and cardiomyocyte genes and a significant increase in the number and the percentage of cardiomyocytes. The increase of the subpopulation correlated with higher numbers of beating foci and beating frequencies. Curcumin on the other hand at 0.4 µM was seen to enhance expression of ectodermal transcripts. Quercetin (2.5 µM) was found to inhibit several developmentally regulated genes.

Keywords
Chemoprevention
Epigallocatechin-3-O-gallate
Quercetin
Curcumin
Embryonic Stem Cells
Embryonic Stem Cell Differentiation
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