IMR Press / FBE / Volume 3 / Issue 4 / DOI: 10.2741/E326

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Na+, K+-ATPase genes are down-regulated during adipose stem cell differentiation

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1 Laboratorio de Biologia del Desarrollo, Departamento de Bioquimica y Biologia Molecular, Universidad de La Laguna, Av. Astrofisico Sanchez s/n. 38201 La Laguna, Tenerife, Spain
2 Division of Veterinary Medicine, School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire, LE12 5RD, United Kingdom

*Author to whom correspondence should be addressed.

Academic Editor: Ali Mobasheri

Front. Biosci. (Elite Ed) 2011, 3(4), 1229–1240; https://doi.org/10.2741/E326
Published: 1 June 2011
(This article belongs to the Special Issue Current trends in cartilage and mesenchymal stem cell biology)
Abstract

The expression of Na+, K+-ATPase alpha and beta subunits isoforms, FXYD2 and FXYD7 were studied in rat adipose stem cell (ASC) by qRT-PCR and immunofluorescence. ASCs were able to differentiate to chondrocytes or adipocytes. All studied genes were expressed in freshly isolated ASCs and in all passages checked. Immunostaining for alpha1 isoform was found in plasma membrane and nuclear envelope, alpha2 signal was lower and alpha3 staining was variable among cells. Beta isoforms signal was abundant and displayed an isoform-specific picture. Staining for FXYD7 was homogeneous in plasma membrane and cytosol. Chondrocytes differenciated from ASC showed identical Na+, K+-ATPase subunits isoforms expression patterns to chondrocytes in cartilage. The expression pattern of Na+, K+-ATPase genes in ASCs exhibits a unique phenotypic signature that implies functional differences in Na+ and K+ transport rates. Furthermore, this phenotypic signature may also be used as a complementary marker for studies of mesenchymal stem cell differentiation. We propose a possible 'moonlighting' role of Na+, K+-ATPase beta isoforms that could be essential for the study of mesenchymal stem cell function and differentiation.

Keywords
Na+
K+ -ATPase
Adipose stem cell
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