IMR Press / FBE / Volume 3 / Issue 4 / DOI: 10.2741/E324

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Developmental patterns of PPAR and RXR gene expression during spermatogenesis
Show Less
1 Department of Anatomy and Neurobiology, Morehouse School of Medicine, Atlanta, GA, 30310-1495
2 Cooperative Reproductive Science Research Center, Morehouse School of Medicine, Atlanta, GA, 30310-1495
3 Department of Obstetrics and Gynecology, Morehouse School of Medicine, Atlanta, GA, 30310-1495
4 Cardiovascular Center, University of Michigan Medical Center, Ann Arbor, MI 48105
5 Department of Biology, University of Texas, San Antonio, TX 78249
6 Department of Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA 15261
7 School of Molecular Biosciences, Washington State University, Pulliman, WA 99164-4060

*Author to whom correspondence should be addressed.

Academic Editor: Indrajit Chowdhury

Front. Biosci. (Elite Ed) 2011, 3(4), 1209–1220; https://doi.org/10.2741/E324
Published: 1 June 2011
(This article belongs to the Special Issue Recent progress in reproductive biology)
Abstract

Members of the family of nuclear receptors that include peroxisome proliferator-activated receptors (PPARs) and retinoid X receptors (RXRs) are important mediators of selective gene activation during development and cellular differentiation. In this study, developmentally-specific PPAR and RXR patterns of expression that occur in somatic and germ cell populations in the testis were determined using quantative real-time PCR (qRT-PCR) studies on RNAs that were isolated from StaPut purified mouse germ cells and primary rat Sertoli cells. These qRT-PCR studies indicate that transcripts encoding the PPAR-Alpha (α), -Beta (β), and -Gamma (γ) and RXR - Alpha (α), -Beta (β), and –Gamma (γ) are developmentally expressed in both differentiating germ and Sertoli cells. In further experiments aimed at deciphering the physiological role that PPAR-Gamma (γ) plays in Sertoli cells, 15-day primary rat Sertoli cells were infected with recombinant adenoviral vectors containing PPAR-Gamma (γ) cDNA and PPAR-Gamma (γ) RNAi constructs. Affymetrix microarray analysis and qRT-PCR validation studies using total RNA isolated from these transfected cells indicated that PPAR-Gamma (γ) regulates the pattern of expression of key lipid metabolic genes in Sertoli cells.

Keywords
Spermatogenesis
Gene Expression
Nuclear Receptors
Testis
Share
Back to top