IMR Press / FBE / Volume 3 / Issue 2 / DOI: 10.2741/E271

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
5-azacytidine enhances proliferation in transplanted rat fetal epiglottis
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1 University Department of Dermatology and Venereology, Zagreb University Hospital Center and School of Medicine, University of Zagreb, Croatia
2 Department of Histology and Embryology, School of Medicine University of Zagreb, Croatia
3 Department of Biology, School of Medicine University of Zagreb, Croatia

*Author to whom correspondence should be addressed,

Front. Biosci. (Elite Ed) 2011, 3(2), 581–590; https://doi.org/10.2741/E271
Published: 1 January 2011
Abstract

Fetal rat epiglottis and its developmental potential in ectopic transplants under the influence of the epigenetic drug was investigated. Epiglottises from 17-days-old rat embryos were transplanted under kidney capsules of adult rats for 14 days. 5-azacytidine (5mg/kg) was injected intraperitoneally during first three days and controls were sham treated. TEM, immunohistochemical detection and quantitative stereological analysis of the Proliferating Cell Nuclear Antigen (PCNA) expression (numerical density Nv) were performed. Typical chondroblasts with long surface processes and sparse lipid droplets were found in fetal epiglottis and chondrocytes with shorter processes, numerous lipid droplets and elastic fibers in adult. PCNA was expressed in almost all cells of the fetal epiglottis while in the adult it was expressed in minority of cells. In transplants, differentiation progressed towards the differentiation found in the adult. Application of 5-azacytidine increased the capacity for proliferation (Nv PCNA) in comparison to controls but no difference in differentiation was observed. Data about the developmental potential and induction of proliferation in mammalian epiglottis by epigenetic modulation is of importance for regenerative medicine.

Keywords
Rat
Fetus
Epiglottis
Transplantation
PCNA
5-Azacytidine
Epigenetics
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