IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E228

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Aberrant methylation as a main mechanism of TSGs silencing in PTC
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1 Department of Molecular Bases of Medicine, Medical University of Lodz, Pomorska St. 251, 92-213 Lodz, Poland
2 Department of General, Oncological and Endocrine Surgery, Medical University of Lodz, Polish Mother’s Memorial Hospital – Research Institute, Rzgowska St. 281/289, 93-338 Lodz, Poland
3 Department of General and Oncological Surgery, Medical University of Lodz, Parzeczewska St. 35, 95-100 Zgierz, Poland

*Author to whom correspondence should be addressed.


Front. Biosci. (Elite Ed) 2011, 3(1), 137–157;
Published: 1 January 2011

In the present study the role of tumour suppressor genes (TSGs) hypermethylation and genetic instability of LOH/MSI type in thyroid tumorigenesis was assessed. Expression, methylation status and presence of LOH/MSI were analyzed for 8 TSGs selected from imprinted (IR) and non-imprinted (NIR) chromosomal regions in papillary thyroid carcinomas (PTCs) and nodular goitres (NGs). The results show that methylation-induced gene silencing occurs at an early step of thyroid carcinogenesis and involves multiple genes. Genetic changes of LOH/MSI type are less frequent. In PTC samples, the lack of significant differences in the frequency of LOH in IR and NIR suggests that it is not a key mechanism changing the pattern of gene expression. Co-methylation observed both in NG and PTC raises a possibility that, in thyroid tissue, methylation-induced silencing may occur not only in malignant transformation but also in functional context. We did not recognize any of the studied TSGs – in regard to aberrant methylation status or LOH/MSI frequency – as a selective molecular marker in thyroid tumorigenesis.

Papillary thyroid carcinoma
Nodular goitre
Tumour suppressor gene
Imprinted region
Non-imprinted region
Methylation pattern
Methylation index
Methylation-specific PCR
Bisulfite-specific PCR
Realtime PCR
Gene expression
Loss of heterozygosity
Microsatellite instability
Fractional allele loss
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