IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E215

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Postprandial transcriptome associated with virgin olive oil intake in rat liver
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1 Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Veterinaria, Instituto Aragones de Ciencias de la Salud (Universidad de Zaragoza- Salud del Gobierno de Aragon), Spain
2 Departamento de Patologia Animal, Facultad de Veterinaria, Universidad de Zaragoza, Spain
3 CIBER de Fisiopatologia de la Obesidad y Nutricion, Instituto de Salud Carlos III, Spain

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2011, 3(1), 11–21;
Published: 1 January 2011

Liver has been proposed as a gatekeeper that regulates postprandial lipemia and a potential target for regulation by acute intake of virgin olive oil. To characterize the hepatic gene expression response to a fat gavage, male rats were fed a bolus of 5 ml of extra-virgin olive oil and the hepatic mRNA expression analyzed 4 hours later using DNA microarrays. To provide an initial screening of candidate genes, only twenty one with remarkably modified expression between both conditions (signal log2 ratio > 2.5 or < -2.5) were considered and confirmed by quantitative real time PCR. Those that presented biological significance were also analyzed 8 hours after the experimental approach. Hepatic A2m Slc13a5 and Nrep mRNA expressions were found significantly changed in both studied conditions and showed the highest significant associations with postprandial plasma triglycerides and lack of association with basal triglyceridemia. These results highlight new gene regulation in liver by postprandial triglyceridemia and will help to understand the complex human pathology providing the involvement of hepatic proteins and new strategies to cope with postprandial metabolism.

Postprandial triglyceridemia
olive oil
hepatic gene expression
DNA microarrays
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