IMR Press / FBE / Volume 2 / Issue 2 / DOI: 10.2741/E111

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Detailed proteomic analysis on DM: insight into its hypoallergenicity
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1 Neonatal Unit, Department of Paediatric and Adolescence Science, University of Turin, Italy
2 Department of Analytical Chemistry, University of Turin, Italy
3 Department. of Paediatric and Adolescence Science, Regina Margherita Children's Hospital, Turin, Italy
4 CNR-Institute of Science of Food Production, Turin, Italy
5 Biochemical and Clinical Laboratory, San Martino Hospital, Genoa, Italy

*Author to whom correspondence should be addressed.


Front. Biosci. (Elite Ed) 2010, 2(2), 526–536;
Published: 1 January 2010

Successful therapy in cow milk (CM) protein allergy rests upon completely eliminating CM proteins from the child's diet: it is thus necessary to provide a replacement food. Donkey milk (DM) has recently aroused scientific and clinical interest, above all among paediatric allergologists. A deeper knowledge of proteins in DM is necessary to evaluate the immunological and physiological properties of this natural substitute for cow's milk. The paper offers a detailed comparative analysis among the protein fractions of DM, CM and human milk, following an extensive proteomic study of the casein and whey proteins of DM performed by narrow pH range 2-DE. The detailed protein composition and structural features reported in this study provide insight into the molecular reasons for the hypoallergenicity of DM. Whole DM might constitute a valid substitute of CM in feeding children with CM protein allergy and it might also constitute the basis for formulas suitable for allergic subjects in the first year of life.

Fatty Acids
Milk Proteins
Mass Spectrometry
Nutritional Value
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