IMR Press / FBE / Volume 14 / Issue 2 / DOI: 10.31083/j.fbe1402011
Open Access Original Research
Culturomics remains a highly valuable methodology to obtain rare microbial diversity with putative biotechnological potential from two Portuguese salterns
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1 Department of Biology, Faculdade de Ciências da Universidade do Porto (FCUP), Rua do Campo Alegre s/n, 4169-007 Porto, Portugal
2 Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Terminal de Cruzeiros do Porto de Leixões, Av. General Norton de Matos s/n, 4450-208 Matosinhos, Portugal
3 Instituto de Ciências Biomédicas Abel Salazar (ICBAS), Rua de Jorge Viterbo Ferreira 228, 4050-313 Porto, Portugal
*Correspondence: malmeida@ciimar.up.pt (Eduarda Almeida)
Academic Editor: Yiannis Kourkoutas
Front. Biosci. (Elite Ed) 2022, 14(2), 011; https://doi.org/10.31083/j.fbe1402011
Submitted: 30 November 2021 | Revised: 13 January 2022 | Accepted: 18 January 2022 | Published: 8 April 2022
Copyright: © 2022 The Author(s). Published by IMR Press.
This is an open access article under the CC BY 4.0 license.
Abstract

Background: The high salt concentration is the major factor limiting microbial growth at salterns, along with solar radiation, temperature, and pH. These environmental factors play key roles in the acquisition of unique genetic adaptations for the survival of microorganisms in salterns, which can result in the production of interesting secondary metabolites. The main goal of the present work was to isolate and compare the culturable microbiota from two geographically distant salterns in Portugal and access their biotechnological potential. Methods: Culturomics approaches using different culture media were applied for microbial isolation. All isolates were identified either by 16S rRNA or ITS genes sequencing, and their biotechonological potential was assessed by PCR. Results: Overall, 154 microbial isolates were recovered that were phylogenetically assigned to 45 taxa from 9 different phyla. From these, 26 isolates may represent putative new taxa. The predominant genera obtained were Penicillium (41 isolates, 26.6%), Streptomyces (13 isolates, 8.4%) and Sinomicrobium (11 isolates, 7.1%). Moreover, the polyketide synthase I gene was present in 64 isolates, the nonribosomal peptide synthethase gene in 16 isolates, and both genes in 23 isolates. Conclusions: This study adds up valuable knowledge on the culturable microbiota of Portuguese salterns and on its potential for production of secondary metabolites. In the long run, this study provides a widely diverse microbial collection for future works. Data public repository: All DNA sequences were deposited in the GenBank database at National Centre for Biotechnology Information (NCBI) web platform under accession numbers OK169439-OK169485, OK216020-OK216124, OK287059 and OK326927.

Keywords
salterns
microbial isolation
microbial diversity
culturomics
bioactive potential
molecular screening
polyketide synthase I
nonribosomal peptide synthethase
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