IMR Press / FBE / Volume 1 / Issue 2 / DOI: 10.2741/E53

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Nuclear localization of ISWI ATPase Smarca5 (Snf2h) in mouse
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1 Pathological Physiology and Center of Experimental Hematology, First Faculty of Medicine, Charles University, Prague, Czech Republic
2 First Medical Department, General Faculty Hospital, Prague, Czech Republic
3 Cell Biology, Albert Einstein College of Medicine, Bronx, New York, NY
Front. Biosci. (Elite Ed) 2009, 1(2), 553–559;
Published: 1 June 2009

Nucleosome movement is, at least in part, facilitated by ISWI ATPase Smarca5 (Snf2h). Smarca5 gene inactivation in mouse demonstrated its requirement at blastocyst stage; however its role at later stages is not completely understood. We herein determined nuclear distribution of Smarca5 and histone marks associated with actively transcribed and repressed chromatin structure in embryonic and adult murine tissues and in tumor cells. Confocal microscopy images demonstrate that Smarca5 is localized mainly in euchromatin and to lesser extent also in heterochromatin and nucleoli. Smarca5 heterozygous mice for a null allele display decreased levels of histone H3 modifications and defects in heterochromatin foci supporting role of Smarca5 as a key regulator of global chromatin structure.

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