IMR Press / EJGO / Volume 7 / Issue 1 / pii/1986001

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with S.O.G.

Original Research

Intracellular localization and serological identification of a HSV-2 protein in cervical cancer

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1 Department of Obstetrics and Gynecology, University of Bologna School of Medicine, Italy
2 Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, USA
3 Divisions of Biophysics and Comparative Medicine, The Johns Hopkins Medical Institutions, Baltimore Maryland, USA
Eur. J. Gynaecol. Oncol. 1986, 7(1), 1–12;
Published: 10 February 1986

Exfoliated atypical cells from US and Italian patients with cervical intraepithelial neoplasia or invasive cancer stained in indirect immunofluorescence with a monoclonal antibody to a high MW HSV-2 protein (ICP10). The proportion of staining cells increased as a function of the severity of the cervical anaplasia. In the majority of cases with mild or moderate dysplasia staining was restricted to the cytoplasm while atypical cells from 6 of 7 cases with marked dysplasia and 7 of 7 cases with invasive cancer evidenced also nuclear staining. Normal exfoliated cells did not stain even when obtained from tissue adjacent to the tumor mass. Patients with ICP10 positive atypical cells had antibody to ICP10 as determined by the Western blot assay. Serologic analyses indicated that there was a good correlation between: (i) the Western blot and the CF assay previously used to detect antibody to AG-4 (Aurelian, et al. Cancer, 48, 455, 1981) and (ii) cervical anaplasia and ICP10 seropositivity. A similar correlation with cervical anaplasia was not observed with respect to antibody to two other viral proteins (ICP12/14 and 68K).

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