Objective: To investigate the infection status of human papillomavirus (HPV) in the cervix of women in Xi’an of China, the genotyping characteristics and the correlation with the results of TCT (Thinprep Cytology Test), in order to provide the basis for the prevention and treatment of cervical lesions in Xi’an of China. Methods: The data of 9174 women who were screened by HPV and TCT in the First Affiliated Hospital of Xi’an Jiaotong University from June 2017 to June 2019 were collected and analyzed statistically. Results: Among 9174 patients, 1045 (10.76%) patients were HPV positive, and the highest infection rate reached 12.26% (116/1045) over 60 years old. In HPV positive patients, the infection rate of single genotype (76.56%, 800/1045) was higher than that of multiple genotype (23.44%, 245/1045) (P 0.01). The top four genotypes were 52, 53, 58 and 16, accounting for 16.24% (214/1318), 11.31% (149/1318), 11.08% (146/1318), and 11.00% (145/1318), respectively. The positive rate of TCT was 10.61% (26/345) in patients with multiple HPV infection and 9.25% (74/800) in patients with single HPV infection. Among the 100 patients with positive HPV and TCT, the top four genotypes were 58, 52, 53 and 16, which were consistent with the overall HPV infection. Limitations and Conclusion: The main subtype of HPV infection in physical examination of women in Xi’an is 52, 58, 53 and 16. This work is valuable for achieving early detection and treatment and thus reducing the incidence of cervical cancer in this area. The combined detection of HPV typing and TCT can improve the effectiveness of cervical disease screening.